Recombinant Human CD68/SR-D1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10551-SR

R&D Systems, part of Bio-Techne
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10551-SR-050
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Key Product Details

Source

CHO

Accession #

P34810.2

Conjugate

Unconjugated

Applications

Bioactivity

View all CD68/SR-D1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD68/SR-D1 protein
Asn22-Ser319, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn22

Predicted Molecular Mass

32 kDa

SDS-PAGE

70-100 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human S100A8 (Catalog # 9876-S8) is immobilized at 10 µg/mL (100 µL/well), it binds to Recombinant Human CD68/SR‑D1 His-tag with an ED50 of 5-30 μg/mL.

Scientific Data Images for Recombinant Human CD68/SR-D1 His-tag Protein, CF

Recombinant Human CD68/SR-D1 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human CD68/SR-D1 His-tag Protein (Catalog # 10551-SR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing broad diffusive bands centered at 70-100 kDa.

Formulation, Preparation and Storage

10551-SR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD68/SR-D1

CD68, also called Scavenger Receptor D1 (SR-D1) and LAMP-4, is a heavily glycosylated type I transmembrane glycoprotein that belongs to the LAMP family comprised of five lysosome-specific protein members including LAMP-1, LAMP-2, dendritic cell (DC)-LAMP (also known as CD208 and LAMP-3), and brain and dendritic cell-associated (BAD)-LAMP (also known as LAMP-5) (1, 2). CD68 expression represents the classical immunohistology marker molecule for human monocytes and tissue resident macrophages (3). It is mainly associated with endosomal/lysosomal compartment and serves as a scavenger receptor for oxidized LDL (4). In contrast to other members of the LAMP family of glycoproteins which are ubiquitously expressed in all cell types, CD68 appears to have a more restricted, cell-type specific pattern of expression (5). Human CD68 is expressed as a 354 amino acid (aa) protein, which includes a 21 aa signal peptide, a 298 aa extracellular domain (ECD), a 25 aa transmembrane segment, and a 10 aa cytoplasmic domain. Within the ECD, human CD68 shares 65% and 67% sequence identity with mouse and rat CD68, respectively. CD68 is a biomarker of rheumatoid arthritis and Hodgkin's lymphoma (6,7). In addition, CD68 on macrophages binds tightly to both S100A8 and S100A9 to enhance the cell immunity and influences the functions of cells through NF-kB/focal adhesion kinase pathway (8, 9).

References

  1. Chistiakov, D. et al. (2016) Lab. Invest. 97:4.
  2. Holness, C.L. and Simmons, D.L. (1993) Blood 81:1607.
  3. O’Reilly D. et al. (2007) Genomics 90:3.
  4. Ramprasad, M.P. et al. (1996) Proc. Natl. Acad. Sci. U. S. A. 93:14838.
  5. Gottfried, E. et al. (2008) Scand. J. Immunol. 67:453.
  6. Bresnihan, B. et al. (2009) J. Rheumatol. 36:1800.
  7. Touati, M. et al. (2015) Leuk. Lymphoma 56:332.
  8. Wang, H. et al. (2014) Cancer Lett. 345:91.
  9. Okada, K. et al. (2016) J. Leukoc. Biol. 100:1093.

Alternate Names

CD68, gp110, Macrosialin, SCARD1, SR-D1, SRD1

Entrez Gene IDs

968 (Human); 12514 (Mouse); 287435 (Rat)

Gene Symbol

CD68

UniProt

P34810.2

Additional CD68/SR-D1 Products

Product Documents for Recombinant Human CD68/SR-D1 His-tag Protein, CF

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Product Specific Notices for Recombinant Human CD68/SR-D1 His-tag Protein, CF

For research use only

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