Recombinant Human CEACAM-16 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9885-CM

R&D Systems, part of Bio-Techne
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9885-CM-050
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Key Product Details

Source

CHO

Accession #

Q2WEN9

Conjugate

Unconjugated

Applications

Bioactivity

View all CEACAM-16 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CEACAM-16 protein
Met1-Gly425, with a C-terminal HA-tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu21

Predicted Molecular Mass

45 kDa

SDS-PAGE

56-73 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9885-CM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-16

Carcinoembryonic Antigen-related Cell Adhesion Molecule 16 (CEACAM-16), or CEAL2, is part of the CEA protein family consisting of CEACAMs and the pregnancy‑specific glycoproteins (PSGs). Both CEACAM and PSG molecules have been identified in humans and belong to the much larger glycosylphosphatidylinositol (GPI)-linked immunoglobulin (Ig) superfamily (1, 2). Unique to the CEA family, CEACAM-16 is a secreted molecule lacking a recognizable transmembrane domain or GPI anchor (3). Mature human CEACAM-16 is 405 amino acids (aa), containing 2 IgC2-like domains and 2 IgV-like domains. CEACAM-16 is one of only five conserved CEACAMs among mice, rats, and humans (2). Mature human CEACAM-16 shares 90% and 89% aa identity with mouse and rat CEACAM‑16, respectively. Originally discovered as a biomarker for colorectal cancer (4), CEACAMs have now been associated with numerous intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (5-7). CEACAM-16 is specifically expressed in the inner ear and has been shown to play a critical role in hearing. CEACAM-16 has been identified as a binding partner for alpha tectorin and specific mutations in CEACAM-16 have been linked to autosomal dominant nonsyndromic deafness (ADNSHL) (3, 8).

References

  1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
  2. Zebhauser R. et al. (2005) Genomics 86:566.
  3. Zheng, J. et al. (2011) PNAS. 108(10):4218.
  4. Gold P. and Freedman S.O. (1965) J Exp Med 122:467.
  5. Obrink, B. (1997) Curr Opin Cell Biol 9:616.
  6. Horst, A.K. and Wagener, C. (2004) Handb Exp Pharmacol 283.
  7. Kuespert K et al. (2006) Curr Opin Cell Biol. 18(5):565.
  8. Wang, H. et al. (2015) J Hum Genet. 60(3):119.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 16

Alternate Names

CEAL2, DFNA4B

Entrez Gene IDs

388551 (Human); 330483 (Mouse); 292700 (Rat)

Gene Symbol

CEACAM16

UniProt

Q2WEN9

Additional CEACAM-16 Products

Product Documents for Recombinant Human CEACAM-16 Protein, CF

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Product Specific Notices for Recombinant Human CEACAM-16 Protein, CF

For research use only

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