Recombinant Human CEACAM-19 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9397-CM

R&D Systems, part of Bio-Techne
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9397-CM-050
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Key Product Details

Source

HEK293

Accession #

Q7Z692-1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CEACAM-19 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CEACAM-19 protein
Human CEACAM-19
(Ala33-Gly157)
Accession # Q7Z692-1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala33

Predicted Molecular Mass

40 kDa

SDS-PAGE

42-56 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line.
The ED50 for this effect is 0.4-2.4 μg/mL

Scientific Data Images for Recombinant Human CEACAM-19 Fc Chimera Protein, CF

Recombinant Human CEACAM-19 Fc Chimera Protein Bioactivity

Recombinant Human CEACAM-19 Fc Chimera Protein Bioactivity

Immobilized Recombinant Human CEACAM-19 (Catalog # 9397-CM) enhances L Cells mouse fibroblasts adhesion. The ED50 for this effect is 0.4-2.4 μg/mL.

Formulation, Preparation and Storage

9397-CM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-19

Carcinoembryonic antigen-related cell adhesion molecule-19 (CEACAM-19), also known as CEA-like gene 1 (CEAL1), is a member of the CEACAM subfamily of glycoproteins in the immunoglobulin (Ig) superfamily. Mature human CEACAM-19 consists of a 125 amino acid (aa) extracellular domain, a 21 aa helical transmembrane domain, and a 122 aa cytoplasmic domain (1,2). The extracellular domain contains an Ig-like domain as well as a glycosylation site, while the cytoplasmic domain is predicted to contain at least one immunoreceptor tyrosine-based activation motif (ITAM) (3,4). The extracellular domain of human CEACAM-19 shows 71%, 72%, and 78% aa identity to mouse, rat, and bovine CEACAM-19. The CEACAM family of proteins are involved in numerous intercellular-adhesion and intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (5,6). CEACAM-19 expression has been identified in a wide range of tissues including prostate, uterus, fetal brain, mammary and adrenal glands, skeletal muscle, small intestine, and kidney (1). Human CEACAM-19 has been found to be overexpressed in BT-474, BT20, and T47D breast cancer cell lines as well as LNCaP prostate cancer cells, suggesting a role in tumor progression and metastasis (1,3, 7). The overexpression in breast cancer patients is associated with poor prognosis (7).

References

  1. Schorilas, A. et al. (2003) Gene 310:79.
  2. Estiar, M. et al. (2016) Clin Exp Med doi:10.1007/s10238-016-0442-1.
  3. Beauchemin, N. and Arabzadeh, A. (2013) Cancer Metastasis Rev 32 (3-4):643.
  4. Kuespert, K. et al. (2006) Curr Opin Cell Biol 18:565.
  5. Obrink, B. (1997) Curr Opin Cell Biol 9:616.
  6. Horst, AK. and Wagener, C. (2004) Handb Exp Pharmacol 283.
  7. Michaelidou, K. et al. (2013) Int J Oncol 42:1770.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 19

Alternate Names

CEACAM19, CEAL1

Entrez Gene IDs

56971 (Human); 319930 (Mouse); 680640 (Rat)

Gene Symbol

CEACAM19

UniProt

Q7Z692-1

Additional CEACAM-19 Products

Product Documents for Recombinant Human CEACAM-19 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human CEACAM-19 Fc Chimera Protein, CF

For research use only

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