Recombinant Human CEACAM-21 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9870-CM

R&D Systems, part of Bio-Techne
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9870-CM-050
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Key Product Details

Source

CHO

Accession #

NP_001091976

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CEACAM-21 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CEACAM-21 protein
Human CEACAM-21
(Trp35-Gly240)
Accession # NP_001091976		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Trp35

Predicted Molecular Mass

50 kDa

SDS-PAGE

62-72 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Galectin-3 (Catalog # 8259-GA) is immobilized at 1 μg/mL, 100 μL/well, it binds Recombinant Human CEACAM-21 Fc Chimera. The concentration of Recombinant Human CEACAM-21 Fc Chimera that produces 50% of the optimal binding response is
0.07-0.7 μg/mL.

Scientific Data Images for Recombinant Human CEACAM-21 Fc Chimera Protein, CF

Recombinant Human CEACAM-21 Fc Chimera Protein Binding Activity

Recombinant Human CEACAM-21 Fc Chimera Protein Binding Activity

When Recombinant Human Galectin-3 (Human Cell-expressed) (Catalog # 8259-GA) is coated at 1 µg/mL, 100 µL/well, Recombinant Human CEACAM-21 Fc Chimera (Catalog # 9870-CM) binds with an ED50 of 0.07-0.7 µg/mL.
Recombinant Human CEACAM-21 Fc Chimera Protein SDS-PAGE

Recombinant Human CEACAM-21 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human CEACAM-21 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 62 - 72 kDa and 130 - 140 kDa, respectively.

Formulation, Preparation and Storage

9870-CM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: CEACAM-21

Carcinoembryonic antigen-related cell adhesion molecule-21 (CEACAM-21) is a member of the CEACAM subfamily of glycoproteins in the immunoglobulin (Ig) superfamily. The CEACAM-21 gene codes for both the CEACAM-3 and CEACAM-21 proteins and is located on chromosome 19. For CEACAM-21, the gene codes a 258 amino acid (aa) polypeptide consisting of a 206 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 32 aa cytoplasmic domain. The mature ECD contains a single IgV-like domain, common to all members of the CEACAM family, and one IgC2-like domain (1). CEACAM-21 is thought to be a primate-specific gene, and to date, no orthologs have been identified in lower species (2). Originally discovered as a biomarker for colorectal cancer (3), CEACAMs have now been associated with numerous intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (4-6). Although the specific function of the CEACAM-21 protein has yet to be elucidated, CEACAM-21 has been found to be overexpressed in heavy smokers (7) and is associated with COPD susceptibility (8). It is also a candidate gene for schizophrenia in the Jewish population (9). CEACAM family members were identified as the major Galectin-3 receptor candidates on human neutrophils (10). Binding of carbohydrate ligands to CEACAMs may be important in the release of proinflammatory mediators (11, 12).

References

  1. Tchoupa, A. et al. (2014) J Cell Commun Signal 12:27.
  2. Zebhauser, R. et al. (2005) Genomics 86:566.
  3. Gold, P. and Freedman S.O. (1965) J Exp Med 122:467.
  4. Obrink, B. (1997) Curr Opin Cell Biol 9:616.
  5. Horst, A.K. Wagener, C. (2004) Handb Exp Pharmacol 283.
  6. Kuespert, K. et al. (2006) Curr Opin Cell Biol 18:565.
  7. Fukuda, I. et al. (1998) J. Med. Syst. 22:89.
  8. Qiu, W. et al. (2011) PLoS ONE 6:e24395.
  9. Alkelai, A. et al. (2012) Int. J. Neuropsycopharmacol. 15:459.
  10. Feuk-Lagerstedt, E. et al. (1999) J. Immunol. 163:5592.
  11. Yoon, J. et al. (2007) J. Immunol. 179:8454.
  12. Schröder, A.K. et al. (2006) Hum Immunol. 67:676.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 21

Alternate Names

CEACAM21, CEACAM3, R29124_1

Entrez Gene IDs

90273 (Human)

Gene Symbol

CEACAM21

UniProt

NP_001091976

Additional CEACAM-21 Products

Product Documents for Recombinant Human CEACAM-21 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human CEACAM-21 Fc Chimera Protein, CF

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