Recombinant Human CEACAM-8/CD66b Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9639-CM

R&D Systems, part of Bio-Techne
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9639-CM-050
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Key Product Details

Source

NS0

Accession #

P31997

Conjugate

Unconjugated

Applications

Bioactivity

View all CEACAM-8/CD66b Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CEACAM-8/CD66b protein
Gln35-Asp320, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln35

Predicted Molecular Mass

32 kDa

SDS-PAGE

67-77 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Galectin‑3 (Catalog # 1154-GA) is coated at 2 μg/mL, 100 μL/well, it binds to Recombinant CEACAM‑8/CD66b with an ED50 of 0.4-2.4 μg/mL.

Scientific Data Images for Recombinant Human CEACAM-8/CD66b Protein, CF

Recombinant Human CEACAM-8/CD66b Protein Bioactivity

Recombinant Human CEACAM-8/CD66b Protein Bioactivity

When Recombinant Human Galectin-3 (Catalog # 1154-GA) is coated at 2 µg/mL, 100 µL/well, it binds to Recombinant CEACAM-8/CD66b (Catalog # 9639-CM) with an ED50 of 0.4-2.4 µg/mL.

Formulation, Preparation and Storage

9639-CM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-8/CD66b

Carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM-8), also known as CGM6 and CD66b, is an approximately 95 kDa member of the human carcinoembryonic antigen (CEA) family that plays a role in cell adhesion, cell migration and pathogen binding (1, 2). CEACAM-8 is anchored to cells by a glycophosphatidylinositol (GPI) motif and is expressed exclusively on granulocytes (3, 4). Human CEACAM-8 is composed of a 34 amino acid (aa) signal peptide, a 29 aa propeptide segment which is removed in the mature form, and a 286 aa CEACAM-8 region containing one V-type and two C2-type Ig-like domains (5). A homologue for CEACAM-8 has not been identified in the mice or rats (1). Activation enhances surface expression of CEACAM-8 and is an activation marker in human neutrophils and eosinophils (1, 6, 7). Engagement of CEACAM-8 by mAb or galectin-3 activated a Src family kinase (lyn), and induced cellular adhesion, superoxide production, and degranulation of eosinophils (1). CEACAM-8 can form both homotypic, and heterotypic bonds with CEACAM-6 through interaction of the V-type Ig-like domains (7-9). Cross-linking of neutrophil CEACAM-8 augments cytokine secretion and beta2 integrin-mediated adhesion (10, 11).

References

  1. Yoon, J. et al. (2007) J. Immunol. 179:8454.
  2. Feuk-Lagerstedt, E. et al. (1999) J. Immunol. 163:5592.
  3. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
  4. Bonsor, D.A. et al. (2015) Proc. Natl. Acad. Sci. USA 112:13561.
  5. Tchoupa, T. et al. (2014) Cell. Commun. Signal. 12:27.
  6. Eades-Perner AM. et al. (1998) Blood 91:663.
  7. Schmidt T. (2012) J Leukoc Biol. 91:791.
  8. Kuroki, M. et al. (2001) J. Leukoc. Biol. 70:543.
  9. Yamanka T et al. (1996) Biochem Biophys Res Commun. 219:842.
  10. Schröder AK et al. (2006) Hum Immunol. 67:676.
  11. Skubitz KM et al. (1996) J Leukoc Biol. 60:106.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 8

Alternate Names

CD66b, CD67, CEACAM8, CGM6, NCA-95

Entrez Gene IDs

1088 (Human); 102138843 (Cynomolgus Monkey)

Gene Symbol

CEACAM8

UniProt

P31997

Additional CEACAM-8/CD66b Products

Product Documents for Recombinant Human CEACAM-8/CD66b Protein, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Human CEACAM-8/CD66b Protein, CF

For research use only

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