Recombinant Human Cerebellin-1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6934-CB

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
6934-CB-025
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

CHO

Accession #

P23435

Conjugate

Unconjugated

Applications

Bioactivity

View all Cerebellin-1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Cerebellin-1 protein
Glu22-Leu193, with an N-terminal HA (YPYDVPDYA) tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr

Predicted Molecular Mass

20 kDa (monomer)

SDS-PAGE

30-35 kDa (monomer) & Oligomer, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Cerebellin-1 is coated at 5 μg/mL, biotinylated recombinant rat NRXN-1 beta binds with an apparent KD < 0.5 nM.

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human Cerebellin-1, immobilized at 3-30 μg/mL, is able to significantly enhance neurite outgrowth.

Formulation, Preparation and Storage

6934-CB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cerebellin-1

Cerebellin-1 (CBLN1) is a 35 kDa secreted glycoprotein in the Cerebellin family of TNF superfamily molecules (1). Cerebellins contain an N‑terminal collagenous domain and a C-terminal TNF/C1q-like domain. Mature human Cerebellin-1 shares 100% aa sequence identity with mouse and rat Cerebellin-1. It is expressed in the cerebellum, the parafascicular nucleus of the thalamus, and in the adrenal cortex and pancreatic islets (2 - 5). Cerebellin-1 forms noncovalent homotrimers that are disulfide-linked into hexamers (6). It can also form hetero-oligomers with Cerebellins-2, -3, and -4 and is required for the secretion of Cerebellin-3 (7 - 9). Cerebellin-1 is subject to proteolysis which can liberate 15-mer or 16-mer bioactive peptides from the C1q domain or remove the regions necessary for trimer or hexamer formation (6). Formation of the hexamer is required for Cerebellin-1’s ability to bind to synaptic structures (9, 10). It binds to postsynaptic densities through direct interactions with the glutamate receptor subunit GluR delta2 and to presynaptic membranes through direct interactions with alpha and beta Neurexins that contain the SS4 insert (11 - 14). The trans-synaptic trimolecular complex of GluR delta2, Cerebellin-1, and Neurexin promotes both presynaptic and postsynaptic development (11, 12). Cerebellin-1 itself is required for synaptic development, maintenance, and function (10, 12 - 15). It can also be internalized by Purkinje cells following secretion by cerebellar granule cells (2, 8, 15). The 16-mer and 15-mer peptides derived from Cerebellin-1 exert multiple endocrine effects including promoting corticosteroid secretion by cortical adrenal cells, inhibiting glucose‑stimulated insulin secretion from pancreatic islets, promoting Neuropeptide Y secretion by the hypothalamus, and decreasing plasma thyroid stimulating hormone (TSH) levels (4, 5, 16).

References

  1. Yuzaki, M. (2011) Curr. Opin. Neurobiol. 21:215.
  2. Wei, P. et al. (2009) Mol. Cell. Neurosci. 41:258.
  3. Kusnoor, S.V. et al. (2010) J. Comp. Neurol. 518:2525.
  4. Rucinski, M. et al. (2009) Int. J. Mol. Med. 23:363.
  5. Strowski, M.Z. et al. (2009) Regul. Pept. 157:19.
  6. Bao, D. et al. (2005) J. Neurochem. 95:618.
  7. Iijima, T. et al. (2007) Eur. J. Neurosci. 25:1049.
  8. Bao, D. et al. (2006) Mol. Cell. Biol. 26:9327.
  9. Matsuda, K. et al. (2009) Eur. J. Neurosci. 29:707.
  10. Ito-Ishida, A. et al. (2008) J. Neurosci. 28:5920.
  11. Matsuda, K. et al. (2010) Science 328:363.
  12. Matsuda, K. and M. Yuzaki (2011) Eur. J. Neurosci. 33:1447.
  13. Uemura, T. et al. (2010) Cell 141:1068.
  14. Joo, J.-Y. et al. (2011) Biochem. Biophys. Res. Commun. 406:627.
  15. Hirai, H. et al. (2005) Nat. Neurosci. 8:1534.
  16. Gardiner, J.V. et al. (2010) Diabetes Obes. Metab. 12:883.

Alternate Names

CBLN1, Cerebellin1, CLN1, Precerebellin

Entrez Gene IDs

869 (Human); 12404 (Mouse); 498922 (Rat)

Gene Symbol

CBLN1

UniProt

P23435

Additional Cerebellin-1 Products

Product Documents for Recombinant Human Cerebellin-1 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Cerebellin-1 Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.