Recombinant Human Cerebellin-2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7044-CB

R&D Systems, part of Bio-Techne
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7044-CB-050
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Key Product Details

Source

CHO

Accession #

Q8IUK8

Conjugate

Unconjugated

Applications

Bioactivity

View all Cerebellin-2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Cerebellin-2 protein
Arg50-Leu224, with an N-terminal HA (YPYDVPDYA) tag

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr

Predicted Molecular Mass

20 kDa

SDS-PAGE

25-35 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Cerebellin-2 is coated at 5 μg/mL, biotinylated recombinant rat NRXN-1 beta binds with an apparent KD <1.5 nM.

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human Cerebellin-2, immobilized at 3-30 μg/mL, is able to significantly enhance neurite outgrowth.

Formulation, Preparation and Storage

7044-CB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cerebellin-2

Cerebellin-2 (CBLN2) is an approximately 25 kDa N-glycosylated protein in the Cerebellin family of TNF superfamily molecules (1). Cerebellins contain a collagenous domain and a C-terminal TNF/C1q-like domain. Human Cerebellin-2 consists of this 175 amino acid (aa) region following an N-terminal 28 aa potential cytoplasmic domain and a 21 aa potential transmembrane segment. Within the Cerebellin-like region, human Cerebellin-2 shares 99% aa sequence identity with mouse, rat, and chick Cerebellin-2 (2‑4). It is expressed in multiple regions of the nervous system including the neocortex, olfactory bulb, thalamus, hypothalamus, midbrain (5), preganglionic sensory nerves, dorsal root ganglia, and sensory and motoneurons of the spinal cord (4, 6) as well as in the zona glomerulosa of the adrenal cortex (7). Cerebellin-2 forms cell associated homo-oligomers and heteromers with Cerebellins-1, -3, and -4 [Iijima 1049]. It binds with high affinity to alpha and beta Neurexin 1 isoforms that contain the splice site 4 insert (+SS4) and more weakly to Neurexin 2 beta (+SS4) and Neurexin 3 beta (+SS4) (9, 10). These interactions promote presynaptic differentiation in cultures of cortical and cerebellar neurons (9, 10).

References

  1. Yuzaki, M. (2011) Curr. Opin. Neurobiol. 21:215.
  2. Kavety, B. et al. (1994) Brain Res. Mol. Brain Res. 27:152.
  3. Wada, C. and H. Ohtani (1991) Brain Res. Mol. Brain Res. 9:71.
  4. Yang, M. et al. (2010) J. Comp. Neurol. 518:2818.
  5. Miura, E. et al. (2006) Eur. J. Neurosci. 24:750.
  6. Reiner, A. et al. (2011) J. Comp. Neurol. 519:2225.
  7. Rucinski, M. et al. (2005) Int. J. Mol. Med. 15:411.
  8. Iijima, T. et al. (2007) Eur. J. Neurosci. 25:1049.
  9. Joo, J.-Y. et al. (2011) Biochem. Biophys. Res. Commun. 406:627.
  10. Matsuda, K. and M. Yuzaki (2011) Eur. J. Neurosci. 33:1447.

Alternate Names

CBLN2, Cerebellin2

Entrez Gene IDs

147381 (Human); 12405 (Mouse); 291388 (Rat)

Gene Symbol

CBLN2

UniProt

Q8IUK8

Additional Cerebellin-2 Products

Product Documents for Recombinant Human Cerebellin-2 Protein, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Human Cerebellin-2 Protein, CF

For research use only

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