Recombinant Human Chondrolectin His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11059-CL

R&D Systems, part of Bio-Techne
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11059-CL-100
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Key Product Details

Source

NS0

Accession #

Q9H9P2.2

Conjugate

Unconjugated

Applications

Bioactivity

View all Chondrolectin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Chondrolectin protein
Arg22-Asn216, with C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg22

Predicted Molecular Mass

23 kDa

SDS-PAGE

34-40 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Chondrolectin His-tag Protein is immobilized at 1.00 ug/mL (100 µL/well), Recombinant Human IGFBP-5 binds with an ED50 of 5.00-30.0 ng/mL.

Scientific Data Images for Recombinant Human Chondrolectin His-tag Protein, CF

Recombinant Human Chondrolectin His-tag Protein Binding Activity.

When Recombinant Human Chondrolectin His-tag Protein (Catalog # 11059-CL) is immobilized at 1.00 ug/mL (100 µL/well), Recombinant Human IGFBP-5 (875-B5) binds with an ED50 of 5.00󈛂.0 ng/mL.

Recombinant Human Chondrolectin His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human Chondrolectin His-tag Protein (Catalog # 11059-CL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 34-40 kDa.

Formulation, Preparation and Storage

11059-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 1.00 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chondrolectin

Chondrolectin (CHODL), also known as transmembrane protein MT75, is a type I transmembrane protein belonging to the C-type lectin-like domain superfamily. CHODL is predominantly expressed by the vascular muscle of the testis and smooth muscle of the heart and prostate (1) Mature CHODL consists of an extracellular domain (ECD) containing one C-type lectin carbohydrate recognition domain (CRD), a helical transmembrane domain, and a cytoplasmic tail. The ECD of CHODL shares 94% and 95% amino acid sequence identity with mouse and rat CHODL, respectively. Multiple isoforms of CHODL with varying C-terminal deletions are generated as a result of alternative splicing (2,3). CHODL is required for proper neuromuscular junction differentiation and is tightly regulated during early embryonic development (4). Dysregulation of CHODL expression is associated with spinal muscular atrophy in mouse (3). Overexpression of CHODL is associated with enhanced cell growth and invasion in lung cancer, while silencing of CHODL in colorectal cancer is a poor prognostic factor (5,6). Expression of specific CHODL isoforms is associated with T cell maturation (2).

References

  1. Weng, L. et al. (2002) Genomics. 80:62.
  2. Weng, L. et al. (2003) J. Biol. Chem. 278:19164.
  3. Sleigh, J. et al. (2013) Hum. Mol. Genet. 23:855.
  4. Oprişoreanu, A.M. et al. (2019) Cell Rep. 29:1082.
  5. Masuda, K. et al. (2011) Clin. Cancer Res. 17:7712.
  6. Zhang, X. et al. (2020) J. Cancer. 11:2874.

Alternate Names

C21orf68, CHODL, MT75, PRED12

Entrez Gene IDs

140578 (Human); 246048 (Mouse)

Gene Symbol

CHODL

UniProt

Q9H9P2.2

Additional Chondrolectin Products

Product Documents for Recombinant Human Chondrolectin His-tag Protein, CF

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Product Specific Notices for Recombinant Human Chondrolectin His-tag Protein, CF

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