Recombinant Human Chymase/CMA1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4099-SE
Key Product Details
Source
NS0
Accession #
Structure / Form
Pro form
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Chymase/CMA1 protein
Met1-Asn247, with a C-terminal 10-His tag
Met1-Asn247, with a C-terminal 10-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gly20
Predicted Molecular Mass
27 kDa
SDS-PAGE
38 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, SUC-Ala-Ala-Pro-Phe-AMC.
The specific activity is >80 pmol/min/µg, as measured under the described conditions.
The specific activity is >80 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
4099-SE
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES and NaCl. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Chymase/CMA1
Chymases are a group of chymotrypsin-like serine proteases secreted by mast cells (1). They are synthesized as inactive precursors containing a 2-residue propeptide, which needs to be removed by dipeptidyl peptidase I/cathepsin C for the enzymatic activity (2). Human Chymase encoded by the CMA1 gene is known to be involved in hypertention and heart failure through its ability to convert angiotensin I (Ang I) to angiotensin II (Ang II), which plays a key role in the regulation of arterial pressure (3). In addition, it is also important in physiological and pathological conditions including inflammation, fibrosis and processing of cytokines (4). Therefore, designing a specific inhibitor for Chymase activity has been a pharmacologic strategy to develop therapeutic agents.
References
- Caughey, G.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. ed. p. 1531, Academic Press, San Diego.
- Murakami, M. et al. (1995) J. Biol. Chem. 270:2218.
- Miyazaki, M. and S. Takai (2006) J. Pharmacol. Sci. 100:391.
- Nakajima, M. and N. Naya (2002) Jpn. J. Pharmacol. 90:206.
Alternate Names
CMA1, CYH, MCP3P, Mcpt5, MCT1
Gene Symbol
CMA1
UniProt
Additional Chymase/CMA1 Products
Product Documents for Recombinant Human Chymase/CMA1 Protein, CF
Product Specific Notices for Recombinant Human Chymase/CMA1 Protein, CF
Triton is a registered trademark of Union Carbide Corp.
For research use only
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