Recombinant Human cIAP-2 (HIAP-1) Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 817-P2
Key Product Details
Product Specifications
Source
E. coli-derived human cIAP-2/HIAP-1 protein
| ATVID | 10-His tag | SIEGRA | Human cIAP-2 (Asn2-Ser604) Accession # Q13489 |
| N-terminus | C-terminus | ||
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
N-terminal Sequence Analysis
Ala
Predicted Molecular Mass
71 kDa
SDS-PAGE
73 kDa, reducing conditions
Activity
Measured by its ability to inhibit DEVD-AFC cleavage activity in cell extracts activated by addition of cytochrome c and dATP.
The IC50 for this effect is 25-1000 nM.
Optimal dilutions should be determined by each laboratory for each application.
The IC50 for this effect is 25-1000 nM.
Optimal dilutions should be determined by each laboratory for each application.
Formulation, Preparation and Storage
817-P2
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: cIAP-2/HIAP-1
cIAP-2 (also known as MIHC and HIAP-1) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. cIAP-2 has 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-2 inhibits caspases through the direct interaction of its BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HtrA2/Omi. cIAP-2 is reported to be cleaved by HtrA2/Omi.
References
- Roy, N. et al. (1997) EMBO J. 23:6914.
- Deveraux, Q. et al. (1997) Nature 388:300.
- Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239.
- Srinivasula, S.M. et al. (2003) J. Biol. Chem. 278:31469.
- Yang, Q-H. et al. (2003) Genes Dev. 17:1487.
Alternate Names
BIRC3, cIAP2, HIAP-1, MIHC
Entrez Gene IDs
330 (Human)
Gene Symbol
BIRC3
UniProt
Additional cIAP-2/HIAP-1 Products
Product Documents for Recombinant Human cIAP-2 (HIAP-1) Protein, CF
Product Specific Notices for Recombinant Human cIAP-2 (HIAP-1) Protein, CF
For research use only
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