Recombinant Human CLEC4E Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8995-CL

R&D Systems, part of Bio-Techne
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8995-CL-050
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Key Product Details

Source

CHO

Accession #

Q9ULY5

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CLEC4E Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CLEC4E protein
MD Human IgG1
(Pro100-Lys330)		 IEGR Human CLEC4E
(Arg41-Leu219)
Accession # Q9ULY5
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

47 kDa

SDS-PAGE

54-64 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Trehalose 6, 6'-Dimycolate is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human CLEC4E Fc Chimera that produces 50% of the optimal binding response is approximately 0.05-0.3 μg/mL.

Formulation, Preparation and Storage

8995-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC4E

CLEC4E, also known as Mincle, is an approximately 30 kDa type 2 transmembrane C-type lectin that functions as an activating innate immune receptor (1). Human CLEC4E consists of a 19 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD) that contains the C-type lectin domain (2). Within the ECD, human CLEC4E shares 65% and 68% aa sequence identity with mouse and rat CLEC4E, respectively. CLEC4E is expressed on monocytes, macrophages, and immature dendritic cells (2-5). It associates with CLEC4D/MCL and the gamma chain signaling subunits of Fc receptors (mediated by an Arg residue in the CLEC4E transmembrane segment) (3, 5, 6). Human CLEC4E binds to mycobacterial glycolipids including the immune adjuvant TDM (cord factor), its synthetic analog TDB, and GroMM (3, 4, 7-10). It also binds the nuclear protein SAP130 which can be released from necrotic cells (5) and cholesterol crystals deposited in atherosclerotic plaques (11). Mouse CLEC4E, in contrast, does not appear to interact with TDB, GroMM, or cholesterol crystals (7, 8, 11). CLEC4E ligation triggers phagocytosis and the production of inflammatory chemokines and cytokines (3-6, 8, 10). The fungus Fonsecaea monophora may evade immune clearance through binding to CLEC4E and suppressing IL-12 production and Th1 cell differentiation instead of promoting inflammation (9).

References

  1. Richardson, M.B. and S.J. Williams (2014) Front. Immunol. 5:288.
  2. Matsumoto, M. et al. (1999) J. Immunol. 163:5039.
  3. Ishikawa, E. et al. (2009) J. Exp. Med. 206:2879.
  4. Wells, C.A. et al. (2008) J. Immunol. 180:7404.
  5. Yamasaki, S. et al. (2008) Nat. Immunol. 9:1179.
  6. Lobato-Pascual, A. et al. (2013) Eur. J. Immunol. 43:3167.
  7. Hattori, Y. et al. (2014) J. Biol. Chem. 289:15405.
  8. Ostrop, J. et al. (2015) J. Immunol. 195:2417.
  9. Wevers, B.A. et al. (2014) Cell Host Microbe 15:494.
  10. Ishikawa, T. et al. (2013) Cell Host Microbe 13:477.
  11. Kiyotake, R. et al. (2015) J. Biol. Chem. 290:25322.

Long Name

C-type Lectin Domain Family 4, Member E

Alternate Names

CLECSF9, MINCLE

Entrez Gene IDs

26253 (Human); 56619 (Mouse); 450223 (Rat)

Gene Symbol

CLEC4E

UniProt

Q9ULY5

Additional CLEC4E Products

Product Documents for Recombinant Human CLEC4E Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human CLEC4E Fc Chimera Protein, CF

For research use only

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