Recombinant Human Collagen IV alpha 1/Arresten Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11597-CL

His-tag
R&D Systems, part of Bio-Techne
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11597-CL-050
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Key Product Details

Source

NS0

Accession #

P02462.3

Conjugate

Unconjugated

Applications

Bioactivity

View all Collagen IV alpha 1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Collagen IV alpha 1 protein
Ser1441-Thr1669, with a C-ter 6xHis tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser 1441

Predicted Molecular Mass

26 kDa

SDS-PAGE

22-33 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human Collagen IV alpha 1 His-tag (Catalog # 11597-CL) binds Recombinant Human Integrin alpha1 beta1 (Catalog # 7064-AB) with an ED50 of 85.0-850 ng/mL.

Scientific Data Images for Recombinant Human Collagen IV alpha 1/Arresten Protein, CF

Recombinant Human Collagen IV alpha 1 His-tag Protein Binding Activity.

Recombinant Human Collagen IV alpha 1 His-tag Protein (Catalog # 11597-CL) binds Recombinant Human Integrin alpha1 beta1 (7064-AB) with an ED50 of 85.0-850 ng/mL.

Recombinant Human Collagen IV alpha 1 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human Collagen IV alpha 1 His-tag Protein (Catalog # 11597-CL) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 22-33 kDa.

Formulation, Preparation and Storage

11597-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Collagen IV alpha 1

Type IV collagen is a member of the collagen superfamily.  Unlike other collagens, type IV occurs only in basement membranes. In mammals, type IV collagen has up to 6 genetically distinct alpha chains, designated alpha-1(IV) to alpha-6(IV), each of which can form a triple helix structure with two other chains (1-3).  Each chain contains three domains: a cysteine- and lysine-rich N-terminal domain, a collagenous triple repeat region, and a non-collagenous (NC1) C-terminal domain (4). The cysteine and lysine residues at the amino-terminal domain are essential for interchain crosslinking (5). Arresten, which comprise the C-terminal NC1 domain, has anti-angiogenic activity and inhibits tumor formation (6-7).  Within this region, human Arresten shares 97% and 75% sequence identity with mouse and rat Arresten, respectively.

References

  1. Myllyharju, J. and KI Kivirikko (2004) Trends Genet. 20:33.
  2. Veit, G. et al. (2006) J. Biol. Chem. 281:3494.
  3. Guey, S. and D. Herve (2022) Cereb. Circ. Cogn. Behav. 3:100140.
  4. Hudson, B.G., et al. (2003) N. Engl. J. Med. 348:2543.
  5. Than, M.E. et al. (2002) Proc. Natl. Acad. Sci. USA 99:6607.
  6. Nyberg, P. et al. (2008) Exp. Cell Res. 314:3292.
  7. Colorado, P.C. et al. (2000) Cancer Res. 60:2520.

Alternate Names

Arresten, COL4A1

Entrez Gene IDs

1282 (Human); 12826 (Mouse); 290905 (Rat)

Gene Symbol

COL4A1

UniProt

P02462.3

Additional Collagen IV alpha 1 Products

Product Documents for Recombinant Human Collagen IV alpha 1/Arresten Protein, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Human Collagen IV alpha 1/Arresten Protein, CF

For research use only

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