Recombinant Human Contactin-3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5539-CN

R&D Systems, part of Bio-Techne
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5539-CN-050
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Key Product Details

Source

NS0

Accession #

Q9P232

Conjugate

Unconjugated

Applications

Bioactivity

View all Contactin-3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Contactin-3 protein
Glu20-Pro992, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu20

Predicted Molecular Mass

108.1 kDa

SDS-PAGE

140-150 kDa, reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Optimal neurite outgrowth was observed when neurons were plated on 96-well culture plates that had been pre-coated with 50 µL/well of the recombinant human Contactin-2 solution at 6-24 μg/mL.

Formulation, Preparation and Storage

5539-CN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 300 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Contactin-3

Contactin-3, also known as BIG-1 and PANG, is a 150 kDa member of the TAG/F3 subgroup of Contactin neuronal adhesion proteins (1, 2). Mature human Contactin-3 consists of six immunoglobulin-like domains, a flexible linker region, four fibronectin type III domains, and a GPI anchor (3, 4). Human Contactin-3 shares 92% aa sequence identity with mouse and rat Contactin-3. Contactin-3 is primarily expressed in the brain in discrete regions including the frontal lobe, occipital lobe, cerebellum, amygdala, and hippocampus (5, 6). Alternative splicing in rat may generate a soluble isoform (4). Mouse plasmacytoma cell lines produce multiple transcripts of Contactin-3 due to the presence of IAP proviruses (3). Immobilized Contactin-3 promotes neurite outgrowth from rat hippocampal neurons (4).

References

  1. Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
  2. Denisenko-Nehrbass, N. et al. (2002) J. Physiol. 96:99.
  3. Connelly, M.A. et al. (1994) Proc. Natl. Acad. Sci. 91:1337.
  4. Yoshihara, Y. et al. (1994) Neuron 13:415.
  5. Kamei, Y. et al. (2000) Genomics 69:113.
  6. Yoshihara, Y. et al. (1995) J. Neurobiol. 28:51.

Alternate Names

BIG-1, CNTN3, Contactin3, PANG, PCS

Entrez Gene IDs

5067 (Human); 18488 (Mouse); 54279 (Rat)

Gene Symbol

CNTN3

UniProt

Q9P232

Additional Contactin-3 Products

Product Documents for Recombinant Human Contactin-3 Protein, CF

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Product Specific Notices for Recombinant Human Contactin-3 Protein, CF

For research use only

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