Recombinant Human Corneodesmosin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5725-CN

R&D Systems, part of Bio-Techne
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5725-CN-050
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Key Product Details

Source

NS0

Accession #

AAN70994

Conjugate

Unconjugated

Applications

Bioactivity

View all Corneodesmosin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Corneodesmosin protein
Lys33-Pro528, with a C-terminal 6-His tag (Asp526Asn)

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys33

Predicted Molecular Mass

49.1 kDa

SDS-PAGE

60 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of BUD-8 human fibroblast cells.
When 3 x 104 cells/well are added to recombinant human CDSN coated plates (10 μg/mL, 100 μL/well), approximately 50-80% will adhere after 1 hour at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

5725-CN
Formulation Lyophilized from a 0.2 μm filtered solution in Tris.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

Background: Corneodesmosin

Corneodesmosin, also known as CDSN and the S gene product, is a highly polymorphic secreted glycoprotein that plays an important structural role in the skin (1). It is expressed by differentiated keratinocytes in the corneal layer of the skin and is a major component of corneodesmosomes (2 - 4). It is also expressed in the inner root sheath of hair follicles (5, 6). Corneodesmosome has a high content of glycine, serine, and proline residues that promote its folding into a series of Gly-loop domains (2, 7). Corneodesmosin forms oligomers and associates homophilically to strengthen the adhesion between corneocytes (8, 9). Corneodesmosin-deficient mice exhibit a detachment of the corneal layer of the skin as well as hypotrichosis of the scalp and baldness (6, 10). Corneodesmosin is secreted by keratinocytes as a 52 - 56 kDa molecule which is then subjected to repeated sequential N- and C-terminal proteolysis (11). Species of 46, 43, 36, and 15 kDa are present in corneocytes (7, 11). Cleavage of the N-terminal Gly-loop diminishes Corneodesmosin’s ability to mediate adhesion, and this is a prerequisite for normal desquamation of the skin (8, 9). Reduced proteolysis of Corneodesmosin in psoriasis lesions is associated with the persistence of corneodesmosomes and scale retention (12). Premature truncation of Corneodesmosin is associated with hypotrichosis of the scalp (13).

References

  1. Pierard, G.E. et al. (2000) Int. J. Mol. Med. 6:217.
  2. Zhou, Y. and D.D. Chaplin (1993) Proc. Natl. Acad. Sci. 90:9470.
  3. Haftek, M. et al. (1997) Br. J. Dermatol. 137:864.
  4. Simon, M. et al. (1997) J. Biol. Chem. 272:31770.
  5. Mils, V. et al. (1992) J. Histochem. Cytochem. 40:1329.
  6. Matsumoto, M. et al. (2008) Proc. Natl. Acad. Sci. 105:6720.
  7. Guerrin, M. et al. (1998) J. Biol. Chem. 273:22640.
  8. Jonca, N. et al. (2002) J. Biol. Chem. 277:5024.
  9. Caubet, C. et al. (2004) J. Invest. Dermatol. 122:747.
  10. Leclerc, E.A. et al. (2009) J. Cell Sci. 122:2699.
  11. Simon, M. et al. (2001) J. Biol. Chem. 276:20292.
  12. Simon, M. et al. (2008) Br. J. Dermatol. 159:77.
  13. Levy-Nissenbaum, E. et al. (2003) Nat. Genet. 34:151.

Alternate Names

CDSN, D6S586E, HTSS

Entrez Gene IDs

1041 (Human); 386463 (Mouse)

Gene Symbol

CDSN

UniProt

AAN70994

Additional Corneodesmosin Products

Product Documents for Recombinant Human Corneodesmosin Protein, CF

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Product Specific Notices for Recombinant Human Corneodesmosin Protein, CF

For research use only

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