Recombinant Human Creatine Kinase MM/CKMM Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9070-CK

R&D Systems, part of Bio-Techne
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9070-CK-050
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Key Product Details

Source

E. coli

Accession #

P06732

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Creatine Kinase MM/CKMM Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Creatine Kinase MM/CKMM protein
Pro2-Lys381, with N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

44 kDa

SDS-PAGE

43 kDa, reducing conditions

Activity

Measured by its ability to phosphorylate creatine.
The specific activity is >1,900 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

9070-CK
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and TCEP.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Creatine Kinase MM/CKMM

Creatine kinase (CK) catalyzes the conversion of creatine to phosphocreatine (PCr) by consuming adenosine triphosphate (ATP) and generating adenosine diphosphate (ADP). CK reaction is reversible and thus ATP can be generated from PCr and ADP (1). In tissues and cells that consume ATP rapidly, especially skeletal muscle, but also brain, photoreceptor cells of the retina, hair cells of the inner ear, spermatozoa and smooth muscle, PCr serves as an energy reservoir for the rapid buffering and regeneration of ATP in situ, as well as for intracellular energy transport by the PCr shuttle or circuit (2). Clinically, creatine kinase is assayed in blood tests as a marker of myocardial infarction (heart attack), rhabdomyolysis (severe muscle breakdown), muscular dystrophy, autoimmune myositides and acute renal failure. Creatine kinase M (CKM), especially, is a cytoplasmic enzyme and is an important serum marker for myocardial infarction. It acts as a homodimer (MM type) in striated muscle as well as in other tissues, and forms a heterodimer (MB type) with CKB, a brain isozyme, in the heart (3). The recombinant human CKM is in the MM type and its activity is measured using a phosphatase-coupled method (4).

References

  1. Trask, R.V. et al. (1988) J. Biol. Chem. 263:17142.
  2. Wallimann, T. et al. (1992) Biochem, J. 281:21.
  3. Wallimann, T. (1994) Curr. Biol.4:42.
  4. Wu, Z.L. (2011) PLoS One 6:e23172.

Long Name

Creatine Kinase, Muscle

Alternate Names

CKM, CKMM, M-CK

Entrez Gene IDs

1158 (Human); 12715 (Mouse); 24265 (Rat)

Gene Symbol

CKM

UniProt

P06732

Additional Creatine Kinase MM/CKMM Products

Product Documents for Recombinant Human Creatine Kinase MM/CKMM Protein, CF

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Product Specific Notices for Recombinant Human Creatine Kinase MM/CKMM Protein, CF

For research use only

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