Recombinant Human CTRP2/C1qTNF2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9436-TN

R&D Systems, part of Bio-Techne
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9436-TN-050
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Key Product Details

Source

HEK293

Accession #

Q9BXJ5

Structure / Form

GS-linked homotrimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CTRP2/C1qTNF2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CTRP2/C1qTNF2 protein
DYKDDDDK
(FLAG tag)		 Human CTRP2/C1qTNF2
(Gly144-Val285)
Accession # Q9BXJ5		 GGGGS Human CTRP2/C1qTNF2
(Gly144-Val285)
Accession # Q9BXJ5		 GGGGS Human CTRP2/C1qTNF2
(Gly144-Val285)
Accession # Q9BXJ5
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp (Flag tag)

Predicted Molecular Mass

49 kDa

SDS-PAGE

54-62 kDa, reducing conditions

Activity

Measured by its ability to induce phospho AMPK activation in C2C12 mouse differentiated myocytes.
100-300 ng/mL of Recombinant Human CTRP2/C1qTNF2 induces phosphorylation of AMPK.

Scientific Data Images for Recombinant Human CTRP2/C1qTNF2 Protein, CF

Recombinant Human CTRP2/C1qTNF2 Protein Bioactivity

Recombinant Human CTRP2/C1qTNF2 Protein Bioactivity

Recombinant Human CTRP2/C1qTNF2 Induces Phosphorylation of AMPK in C2C12 Differentiated Myocytes. C2C12 differentiated myocytes were treated with Recombinant Human CTRP2/C1qTNF2 (Catalog # 9436-TN) for 1 hour prior to Western blot analysis.

Formulation, Preparation and Storage

9436-TN
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTRP2/C1qTNF2

Complement C1q tumor necrosis factor-related protein 2 (C1qTNF2), also known as CTRP-2 or zacrp2 is an approximately 30 kDa member of the C1q family of secreted proteins (1, 2). Like other members of that family, CTRP2 contains a collagen-like region and one C1q-like domain (3). CTRP2 shares 42% amino acids identity with adiponectin at the presumed functional globular domain (4) and is expressed in stromal vascular cells (5). CTRP2 circulates as a trimeric glycoprotein in plasma (6), and can also form homo-oligomer or hetero-oligomer with other CTRPs (7). C1qTNF2 has been shown to phosphorylate AMPK and ACC in skeletal muscle leading to an increase in glycogen accumulation and fatty acid oxidation (8). C1qTNFs are of clinical interest as diagnostic or therapeutic targets in diseases where energy homeostasis is unbalanced such as obesity, insulin resistance, and type 2 diabetes (2).

References

  1. Hosszu, K.K. et al. (2012) Front. Immunol. 5:3.
  2. Seldin, M.M. et al. (2014) Rev. Endocr. Metab. Disord. 15:111.
  3. Kim, K.-Y. et al. (2006) FEBS Lett. 580:3953.
  4. Wong, G.W et al. (2004). Proc. Natl. Acad. Sci. U.S.A. 101:10302.
  5. Schäffler, A. et al. (2012) Trends in Endocrinology and Metabolism. 23:194.
  6. Wong, G.W. et al. (2008) Biochem J. 416:161.
  7. Peterson, J.M.l. et al. (2014) PLOS one 9:e88535.
  8. Schäffler, A. et al. 2007) Trends in Immunology 28:393.

Long Name

C1q And Tumor Necrosis Factor Related Protein 2

Alternate Names

C1QTNF2, zacrp2

Entrez Gene IDs

114898 (Human); 69183 (Mouse); 497886 (Rat)

Gene Symbol

C1QTNF2

UniProt

Q9BXJ5

Additional CTRP2/C1qTNF2 Products

Product Documents for Recombinant Human CTRP2/C1qTNF2 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human CTRP2/C1qTNF2 Protein, CF

For research use only

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