Recombinant Human CTRP9/C1qTNF9 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6537-TN

R&D Systems, part of Bio-Techne
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6537-TN-050
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Key Product Details

Source

NS0

Accession #

AAH40438

Conjugate

Unconjugated

Applications

Bioactivity

View all CTRP9/C1qTNF9 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CTRP9/C1qTNF9 protein
Asn16-Pro333, with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

34 kDa

SDS-PAGE

36-40 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CTRP9/C1qTNF9 is immobilized at 0.5 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human CL-K1/COLEC11 that produces 50% of the optimal binding response is 0.035-0.21 μg/mL.

Scientific Data Images for Recombinant Human CTRP9/C1qTNF9 Protein, CF

Recombinant Human CTRP9/C1qTNF9 Protein Binding Activity

Recombinant Human CTRP9/C1qTNF9 Protein Binding Activity

When Recombinant Human CTRP9/C1qTNF9 (Catalog # 6537-TN) is coated at 0.5 μg/mL, 100 μL/well, Biotinylated Recombinant Human CL-K1/COLEC11 binds with an ED50 of 0.035-0.21 μg/mL.

Formulation, Preparation and Storage

6537-TN
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
Reconstitution
Reconstitute at 250 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTRP9/C1qTNF9

C1qTNF9, also known as CTRP9, is an approximately 40 kDa member of the C1q and TNF-related protein family (1). Like all members of this protein family, C1qTNF9 consists of a short variable region, a collagenous domain that can be hydroxylated, and a C1q-like globular domain (1). Human C1qTNF9 shares 85% amino acid sequence identity with the mouse and rat orthologs. Both the mouse and human C1qTNF9 proteins are expressed in adipose tissue, but the mouse protein has also been detected in the heart, lung, muscle, kidney, testis, lymph node, smooth muscle, prostate, thymus, and uterus (1, 2). They have both also been shown to be secreted as trimers and higher order multimers and also to form hetero-oligomers with Adiponectin (1, 2). Mouse C1qTNF9 can stimulate the phosphorylation of AMPK, Akt, and eNOS (1, 3, 4). Also in mice, C1qTNF9 may have an important role in cardiac and metabolic health. Its expression has a cardioprotective effect following acute myocardial infarction that may be dependent on AMPK activation (4-6). Additionally, transgenic mice overexpressing C1qTNF9 are resistant to high fat diet‑induced obesity (7). This metabolic role may be conserved, since C1qTNF9 serum levels have been shown to inversely correlate with metabolic syndrome in humans (8).

References

  1. Wong, G.W. et al. (2009) FASEB J. 23:241.
  2. Peterson, J.M. et al. (2009) Biochem. Biophys. Res. Commun. 388:360.
  3. Zheng, Q. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:2616.
  4. Kambara, T. et al. (2012) J. Biol. Chem. 287:18965.
  5. Su, H. et al. (2013) Basic Res. Cardiol. 108:315.
  6. Sun, Y. et al. (2013) Circulation 128:S113.
  7. Peterson, J.M. et al. (2013) Am. J. Physiol. Regul. Integr. Comp. Physiol. 305:R522.
  8. Hwang, Y.C. et al. (2013) Int. J. Obes. (Lond) [Epub ahead of print].

Long Name

C1q and Tumor Necrosis Factor Related Protein 9

Alternate Names

AQL1, C1qTNF9

Entrez Gene IDs

338872 (Human); 239126 (Mouse)

Gene Symbol

C1QTNF9

UniProt

AAH40438

Additional CTRP9/C1qTNF9 Products

Product Documents for Recombinant Human CTRP9/C1qTNF9 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human CTRP9/C1qTNF9 Protein, CF

For research use only

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