Recombinant Human CUL4A/RBX1 Neddylated Complex Protein, CF

R&D Systems, part of Bio-Techne | Catalog # E3-441

R&D Systems, part of Bio-Techne
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E3-441-025
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Q13619.3 (CUL4A), P62877.1 (RBX1)

Conjugate

Unconjugated

Applications

Bioactivity

View all CUL4A/RBX1 Complex Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL4A/RBX1 Complex protein
Met1 - Ala759 with a N-terminal 10-His Sumo tag (CUL4A), Met1 - His108 (RBX1)

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

94-104 kDa (neddylated CUL4A), & 12 kDa (RBX1)

Activity

Typical enzyme concentration to support in vitro conjugation will depend on experimental conditions.

Formulation, Preparation and Storage

E3-441
Formulation Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: CUL4A/RBX1 Complex

Cullin-4A (CUL4A) is a core component of multiple cullin-RING type E3 Ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in cell cycle progression, DNA repair and other processes.  In the DCX complex (DDB1-CUL4-X-box) CUL4A serves as a scaffold that organizes the DDB1-X-box recognition subunits with the RBX1 subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  In vivo, the E3 ubiquitin ligase activity of the DCX complex is dependent on neddylation of the cullin subunit, though neddylation may be dispensable for some in vitro reactions.  

References

  1. Baek K., et al. (2020) Nature 578: 461.
  2. Fischer E.S., et al.  (2014) Nature  512: 49.
  3. He Y.J., et al.  (2006) Genes Dev.  20: 2949.
  4. Ito T., et al.  (2010) Science  327: 1345.
  5. Wang H., et al.  (2006) Mol. Cell  22: 383.

Long Name

Cullin-4A/RING Box Protein 1 Complex

Entrez Gene IDs

8451 (Human)

Gene Symbol

CUL4A

UniProt

Q13619.3 (CUL4A), P62877.1 (RBX1)

Additional CUL4A/RBX1 Complex Products

Product Documents for Recombinant Human CUL4A/RBX1 Neddylated Complex Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human CUL4A/RBX1 Neddylated Complex Protein, CF

For research use only

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