Recombinant Human Cystatin D Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1202-PI
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Cystatin D protein
Thr29-Val142, with a C-terminal 10-His tag
Thr29-Val142, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Thr29
Predicted Molecular Mass
15 kDa
SDS-PAGE
15 kDa, reducing conditions
Activity
Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC (Catalog # ES009).
The IC50 value is <8 nM, under the described conditions.
The IC50 value is <8 nM, under the described conditions.
Formulation, Preparation and Storage
1202-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in MES and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 50 mM Tris, pH 7.0. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Cystatin D
Cystatin D is a member of family 2 of the cystatin superfamily (1). In contrast to other members of family 2, Cystatin D has restricted tissue distribution and has been found only in saliva and tears. Two allelic variants (Arg46 and Cys46) are known in the human protein and they are not significantly different in their inhibitory activity against papain and cathepsins B, H, L and S (2). Recombinant Human Cystatin D corresponds to the Arg46 variant. The functions of Cystatin D are largely unknown. However, Cystatin D has been shown to inhibit coronavirus replication at its physiological concentration (0.12‑1.9 μM) and has been suggested to play a protective role against proteases present in the oral cavity (3).
References
- Freije, J.P. et al. (1993) J. Biol. Chem. 268:15737.
- Balbin, M. et al. (1994) J. Biol. Chem. 269:23156.
- Collins, A.R. and A. Grubb (1998) Oral Microbiol. Immunol. 13:59.
Alternate Names
CST5, CSTD
Entrez Gene IDs
1473 (Human)
Gene Symbol
CST5
UniProt
Additional Cystatin D Products
Product Documents for Recombinant Human Cystatin D Protein, CF
Product Specific Notices for Recombinant Human Cystatin D Protein, CF
For research use only
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