Recombinant Human Cystatin S Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1296-PI
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Cystatin S protein
Ser21-Ala141, with a C-terminal 10-His tag
Ser21-Ala141, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser21
Predicted Molecular Mass
15.6 kDa
SDS-PAGE
16 kDa, reducing conditions
Activity
Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC (Catalog # ES008).
The IC50 value is <1.0 µM as measured under the described conditions.
The IC50 value is <1.0 µM as measured under the described conditions.
Formulation, Preparation and Storage
1296-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in MES and NaCl. |
| Reconstitution | Reconstitute at 1 mg/mL in sterile 25 mM MES, 150 mM NaCl, pH 6.5. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Cystatin S
Cystatin S is a member of family 2 of the cystatin superfamily (1). Like cystatins SA and SN, it is produced by the salivary gland and secreted largely in the submandibular/sublingual saliva (2). Unlike cystatins SA and SN, Cystatin S is not a potent inhibitor of cysteine proteases such as papain and some cathepsins (3). Cystatin S is partially phosphorylated at Ser21 and Ser23 in saliva (4). The functions of Cystatin S are largely unknown. However, it is able to bind more calcium and bind more rapidly to carbonated apatite than cystatins SA or SN, indicating that Cystatin S may be involved in the mineral balance of the tooth (3).
References
- Abrahamson, M. (1994) Methods Enzymol. 244:685.
- Baron, A.C. et al. (1999) Oral. Dis. 5:344.
- Baron, A. et al. (1999) Oral Dis. 5:234.
- Isemura, S. et al. (1991) J. Biochem. 110:648.
Alternate Names
CST4
Entrez Gene IDs
1472 (Human)
Gene Symbol
CST4
UniProt
Additional Cystatin S Products
Product Documents for Recombinant Human Cystatin S Protein, CF
Product Specific Notices for Recombinant Human Cystatin S Protein, CF
For research use only
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