Recombinant Human DCIR/CLEC4A Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9784-CI

R&D Systems, part of Bio-Techne
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9784-CI-050
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Key Product Details

Source

NS0

Accession #

Q9UMR7-1

Conjugate

Unconjugated

Applications

Bioactivity

View all DCIR/CLEC4A Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human DCIR/CLEC4A protein
Gln70-Leu237, with an N-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

21 kDa

SDS-PAGE

23-38 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of SW480 human fibroblast carcinoma cells.
The ED50 for this effect is 0.8-6.4 μg/mL.

Scientific Data Images for Recombinant Human DCIR/CLEC4A Protein, CF

Recombinant Human DCIR/CLEC4A Protein Bioactivity

Recombinant Human DCIR/CLEC4A Protein Bioactivity

Recombinant Human DCIR/CLEC4A (Catalog # 9784-CI) supports the adhesion of SW480 human fibroblast carcinoma cells. The ED50 for this effect is 0.8-6.4 μg/mL.

Formulation, Preparation and Storage

9784-CI
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DCIR/CLEC4A

DCIR (Dendritic Cell Immunoreceptor), also known as Lectin-like Immunoreceptor (LLIR), is a type II membrane protein belonging to the C-type lectin domain family and is designated CLEC4A (previously designated CLECSF6). Four transcript variants encoding distinct isoforms have been identified (1, 2). DCIR contains one carbohydrate recognition domain in its C-terminal extracellular domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain (3). Crystal structure identifies the nonterminal disaccharide GlcNAc beta1-2Man as its primary binding epitope (4). Human DCIR consists of 237 amino acids (aa) with a 48 aa cytoplasmic domain, a 21 aa transmembrane region, and a 168 aa extracellular domain. Human DCIR shares approximately 56% amino acid identity with the mouser version and 55% amino acid identity with the rat version of the protein. Besides dendritic cell, DCIR is expressed on B cells, monocytes/macrophages and granulocytes. It acts as a mannose/fucose lectin and interacts with targets of both endogenous and pathogenic origin (5), binding sugars with broad specificity in a calcium-dependent manner (4). DCIR is critically important for the homeostasis of the immune system. DCIR can inhibit B cell receptor mediated calcium mobilization and protein tyrosine phosphorylation through its intracellular ITIM Motif (6, 7). It can interact directly with the HIV-1 virus thus modulate HIV-1 transmission (8). Recent study has demonstrated that DCIR-specific ligands are present on various cancer cell lines and keratinocytes (5).

References

  1. Huang, X. et al. (2001) Biochem. Biophys. Res. Commun. 281:131.
  2. Richard, M. et al. (2002) J. Leukoc. Biol. 71:871.
  3. Bates E.E. et al. (1999) J. Immunol. 163:1973.
  4. Nagae, M. et al. (2016) FEBS Lett. 590:1280.
  5. Bloem, K. et al. (2014) Immunol. Lett. 158:33.
  6. Kanazawa, N. et al. (2002) Dermatol. 118(2):261.
  7. Maruhashi, T. et al. (2015) J. Immunol. 194(12):5681.
  8. Lambert, A. et al. (2008) Blood 112(4):1299.

Long Name

Dendritic Cell Immunoreceptor

Alternate Names

CD367, CLEC4A, CLECSF6, DDB27, LLIR

Entrez Gene IDs

50856 (Human); 26888 (Mouse)

Gene Symbol

CLEC4A

UniProt

Q9UMR7-1

Additional DCIR/CLEC4A Products

Product Documents for Recombinant Human DCIR/CLEC4A Protein, CF

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Product Specific Notices for Recombinant Human DCIR/CLEC4A Protein, CF

For research use only

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