Recombinant Human Desmoglein-4 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9230-DM

R&D Systems, part of Bio-Techne
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9230-DM-100
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Key Product Details

Source

HEK293

Accession #

Q86SJ6

Structure / Form

Disulfide-liked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Desmoglein-4 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Desmoglein-4 protein
Human Desmoglein-4
(Glu24-Ala631)
Accession # Q86SJ6		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu24

Predicted Molecular Mass

94 kDa

SDS-PAGE

95-115 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells.
The ED50 for this effect is 0.4-2.4 μg/mL.

Scientific Data Images for Recombinant Human Desmoglein-4 Fc Chimera Protein, CF

Recombinant Human Desmoglein-4 Fc Chimera Protein Bioactivity

Recombinant Human Desmoglein-4 Fc Chimera Protein Bioactivity

Immobilized Recombinant Human Desmoglein‑4 Fc Chimera (Catalog # 9230-DM) supports the adhesion of A431 human epithelial carcinoma cells. The ED50 for this effect is 0.4-2.4 μg/mL.

Formulation, Preparation and Storage

9230-DM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Desmoglein-4

Desmoglein-4 is an approximately 140 kDa transmembrane glycoprotein in the cadherin family of calcium dependent adhesion molecules. Human Desmoglein-4 is synthesized with a 26 amino acid (aa) propeptide followed by a 582 aa extracellular domain (ECD) with four tandem cadherin-like domains, a 21 aa transmembrane segment, and a 388 aa cytoplasmic domain (1, 2). Within the ECD, human Desmoglein-4 shares 82% aa sequence identity with mouse and rat Desmoglein-4. Alternative splicing generates additional isoform with a substitution in the cytoplasmic domain. Desmoglein-4 is the principal cadherin of desmosomes which form adhesive contacts between epithelial cells (2, 3). It is expressed in hair follicles and differentiated keratinocytes in suprabasal layers of the skin and plays a role in keratinocyte differentiation and adhesion (2, 3). Mutations in human Desmoglein-4 are associated with the development of monilethrix autosomal recessive hypotrichosis (LAH) (2, 4-8). Autoantibodies directed against Desmoglein-3 in the blistering skin disorder pemphigus vulgaris can cross-react with Desmoglein-4 (2, 9, 10).

References

  1. Whittock, N.V. and C. Bower (2003) J. Invest. Dermatol. 120:523.
  2. Kljuic, A. et al. (2003) Cell 113:249.
  3. Bazzi, H. et al. (2006) Differentiation 74:129.
  4. Bazzi, H. et al. (2005) J. Investig. Dermatol. Symp. Proc. 10:222.
  5. Schaffer, J.V. et al. (2006) J. Invest. Dermatol. 126:1286.
  6. Shimomura, Y. et al. (2006) J. Invest. Dermatol. 126:1281.
  7. Zlotogorski, A. et al. (2006) J. Invest. Dermatol. 126:1292.
  8. Kato, M. et al. (2015) J. Invest. Dermatol. 135:1253.
  9. Nagasaka, T. et al. (2004) J. Clin. Invest. 114:1484.
  10. Calkins, C.C. et al. (2006) J. Biol. Chem. 281:7623.

Alternate Names

Cad13, CDGF13, CDHF13, Desmoglein4, DSG4

Entrez Gene IDs

147409 (Human); 16769 (Mouse); 291754 (Rat)

Gene Symbol

DSG4

UniProt

Q86SJ6 (Human)

Additional Desmoglein-4 Products

Product Documents for Recombinant Human Desmoglein-4 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human Desmoglein-4 Fc Chimera Protein, CF

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