Recombinant Human DGCR2 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10161-DG

R&D Systems, part of Bio-Techne
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10161-DG-050
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Key Product Details

Source

CHO

Accession #

P98153

Conjugate

Unconjugated

Applications

Bioactivity

View all DGCR2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human DGCR2 protein
Glu22-Ala349, with an C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu22

Predicted Molecular Mass

38 kDa

SDS-PAGE

40-60 kDa, under reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human DGCR2, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth. 

Scientific Data Images for Recombinant Human DGCR2 His-tag Protein, CF

Recombinant Human DGCR2 His-tag Protein SDS-PAGE

Recombinant Human DGCR2 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human DGCR2 His-tag (Catalog # 10161-DG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-60 kDa.

Formulation, Preparation and Storage

10161-DG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DGCR2

DiGeorge Syndrome Critical Region 2 (DGCR2), also known as IDD, DGS-C, LAN, and SEZ-12, is an adhesion receptor protein located on the long arm of chromosome 22 (1). The DGCR2 gene encodes an integral membrane protein, consisting of an extracellular domain, a single transmembrane region and a cytoplasmic tail (1). The mature extracellular domain (ECD) of DGCR2 contains both a C-type lectin domain and a cysteine-rich region similar to that of the low density lipoprotein receptor (LDLR) (1, 3). The mature ECD of human DGCR2 shares 93% and 92% amino acid sequence identity with mouse and rat, respectively. DGCR2 is expressed during neurodevelopment in human brain tissues (3). Deletion of the 22q11.2 region results in an extremely variable disorder called 22q11.2 deletion syndrome, with a phenotype ranging from very mild symptoms to severe intellectual disability, facial dysmorphism, heart defects, and urogenital abnormalities (4). Recent studies suggest that DGCR2 regulates critical steps of early cortico-genesis possibly through a Reelin-dependent mechanism. Deletion of DGCR2 has a pathogenic impact on cortical formation by reducing protein expression level, and it plays a critical role in vulnerability to schizophrenia (5). Furthermore, expression of DGCR2 together with USP18 gene may serve as a progonostic marker for muscle invasive bladder cancer survival in patients (5).

References

  1. Augusseau, S. et al. (1986) Hum. Genet. 74:206.
  2. Mugikura, S. et al. (2016) Biochem Biophys Rep. 5:120.
  3. Kajiwara, K. et al. (1996) Biochem. Biophyis. Res. Commun. 222:144.
  4. Vaisvilas, M. et al. (2018) Balkan J Med Genet. 21:87.
  5. Molinard-Chenu A. and Dayer A. (2017) Biol. Psych. 83:692.

Long Name

DiGeorge Syndrome Critical Region Gene 2

Alternate Names

KIAA0163, SEZ-12

Entrez Gene IDs

9993 (Human); 13356 (Mouse); 360742 (Rat)

Gene Symbol

DGCR2

UniProt

P98153

Additional DGCR2 Products

Product Documents for Recombinant Human DGCR2 His-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human DGCR2 His-tag Protein, CF

For research use only

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