Recombinant Human DPEP1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11524-DP

R&D Systems, part of Bio-Techne
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11524-DP-020
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Key Product Details

Source

CHO

Accession #

P16444.3

Conjugate

Unconjugated

Applications

Enzyme Activity

View all DPEP1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human DPEP1 protein
Asp17-Ser384, with C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp17

Predicted Molecular Mass

42 kDa

SDS-PAGE

39-49 kDa, under reducing conditions

Activity

Measured by its ability to cleave carnosine ( beta-Ala-L-His) in a two-step assay.
The specific activity is >5 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human DPEP1 His-tag Protein, CF

Recombinant Human DPEP1 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human DPEP1 His-tag Protein (Catalog # 11524-DP) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 39-49 kDa, under reducing conditions.

Formulation, Preparation and Storage

11524-DP
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: DPEP1

Dipeptidase 1 (DPEP1), also referred to as renal peptidase or beta-lactamase, is a zinc-dependent, glycosylated member of the M19 peptidase metallo-dependent hydrolase superfamily that lacks a sequence commonly found in zinc proteases and has poor homology to other metallopeptiases (1). DPEP1 is a homodimeric elongated ellipsoid with a single disulfide bond between the subunits (1) and GPI-anchored to the cell membrane at the brush border of epithelial cells (2). Each DPEP1 monomer contains a zinc-dependent active site, a propeptide, and a GPI anchor at the c-terminus (1). DPEP1 has activity towards several dipeptides and is critical in glutathione and leukotriene metabolism as well as mammalian metabolism of the B-lactam ring of antibiotics (1, 3). DPEP1 expression is upregulated or associated as a marker in various cancers including colon, kidney, and prostate cancer (4-6). It is also upregulated in renal tubular epithelial cells and associated with iron-related ferroptotic cell death in diabetic retinopathy (2, 7) where it may mediate proinflammatory cancer mediatory leukotrienes or serve as a receptor as an adhesion receptor for neutrophil recruitment and impact inflammation (8-10). DPEP1 has been noted as a potential therapeutic marker or target for mestastasis, cancer progression, and disease in the liver and lungs with acute or chronic inflammation (2, 4, 9, 11).

References

  1. Nitanai, Y. et. al. (2002) J. Mol. Biol. 321:177.
  2. Li, Y. et. al. (2024) Int. Immunopharmacol. 133:111955. 
  3. Campbell, B.J. et. al. (1988) Biochim. Biophys. Acta 956:110.
  4. Tachibana, K. et. al. (2017) Biomed. Rep. 6:423.
  5. Xiang, C. et. al. (2023) J. Cell Mol. Med. 27:1947.
  6. Zhu, W. et. al. (2023) Heliyon 9:e18870.
  7. Allison, S.J. (2021) Nat. Rev. Nephrol. 17:707.
  8. Satoh, S. et. al. (1993) Biochim. Biophys. Acta 1172:181. 
  9. Choudhury, S.R. et. al. (2019) Cell 178:1205.
  10. Wang, M. (2022) Nat. Rev. Nephrol. 18:199. 
  11. Tutanov. O.S. et. al. (2023) Extracell. Vesicles Circ. Nucl. Acids. 4:195.

Long Name

Dipeptidase 1

Alternate Names

HRDP, Microsomal Dipeptidase, Renal Dipeptidase

Entrez Gene IDs

1800 (Human)

Gene Symbol

DPEP1

UniProt

P16444.3

Additional DPEP1 Products

Product Documents for Recombinant Human DPEP1 His-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human DPEP1 His-tag Protein, CF

For research use only

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