Recombinant Human DPP9 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 5419-SE
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human DPP9 protein
Arg2-Leu892 with an N-terminal Met and 6-His tag
Arg2-Leu892 with an N-terminal Met and 6-His tag
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
No result obtained
Predicted Molecular Mass
101 kDa
SDS-PAGE
92 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC).
The specific activity is >750 pmol/min/μg, as measured under the described conditions.
The specific activity is >750 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
5419-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: DPP9
DPP9 is a member of the S9b family of serine peptidases (1, 2). It shares 19% amino acid identity with DPP4 and 58% amino acid identity with DPP8. It exhibits post‑proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (3). Unlike DPP4, DPP9 does not appear to be membrane bound and is localized exclusively in the cytoplasm (4). This family of proline-specific dipeptidyl peptidases has been implicated in a variety of diseases including type 2 diabetes, obesity and cancer, and has been a potential target for drug discovery (5, 6).
References
- Olsen, C. and Wagtmann, N. (2002) Gene 299:185.
- Qi, S.Y. et al. (2003) Biochem. J. 373:179.
- Bjelke, J.R. et al. (2006) Biochem. J. 396:391.
- Ajami, K. et al. (2004) Biochim. Biophys. Acta. 1679:18.
- Rosenblum, J.S. and Kozarich, J.W. et al. (2003) Curr. Opin. Chem. Biol. 7:496.
- Van der Veken, P. et al. (2007) Curr. Top. Med. Chem. 7:621.
Long Name
Dipeptidyl peptidase 9
Alternate Names
DPLP9, DPRP2
Gene Symbol
DPP9
UniProt
Additional DPP9 Products
Product Documents for Recombinant Human DPP9 Protein, CF
Product Specific Notices for Recombinant Human DPP9 Protein, CF
For research use only
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