Recombinant Human EDIL3 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 6046-ED
Key Product Details
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human EDIL3 protein
Val17-Glu480 with a C-terminal 6x His
Val17-Glu480 with a C-terminal 6x His
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Val17, Asp24
Predicted Molecular Mass
52 kDa
SDS-PAGE
60-65 kDa, reducing conditions
Activity
Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells.
When 5 x 104 cells/well are added to Recombinant Human EDIL3 coated plates (5 µg/mL, 100 µL/well), >45% will adhere after 60 minutes at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
When 5 x 104 cells/well are added to Recombinant Human EDIL3 coated plates (5 µg/mL, 100 µL/well), >45% will adhere after 60 minutes at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Formulation, Preparation and Storage
6046-ED
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 200 μg/mL in PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: EDIL3
aa 67‑76 found in isoform 1. Mature human EDIL3 shares 96% aa sequence identity with mature mouse EDIL3. The RGD motif of EDIL3 binds alphav beta5 integrin, which, in turn, leads to increased angiogenic transcription factor HoxD3 expression (2). HoxD3activates alphav beta3 and uPA, resulting in the transformation of resting endothelial cells to an angiogenic state (2‑4). EDIL3 becomes quiescent at the time of birth, and is no longer expressed in normal adult tissues. It has been found, however,
re‑expressed in a number of human tumors as well as in ischemic muscles and ischemic brain tissue, which may play an important role in adult angiogenesis (2, 5‑6). EDIL3 promotes adherence and migration of endothelial cells, and acts as an endothelial cell survival agent through up‑regulation of Bcl‑2 expression (7). Exogenous application of EDIL3 has been demonstrated to augment angiogenesis and improve blood flow and tissue function in murine models of hind‑limb ischemia (6, 8), cardiac ischemia (9) and cerebral ischemia (2). EDIL3 has also been shown to be an endogenous inhibitor of inflammatory cell recruitment by interfering with the integrin LFA‑1‑dependent leukocyte‑endothelial adhesion (10).
References
- Hidai, C. et al. (1998) Genes Dev. 12:21.
- Fan, Y. et al. (2008) Brain Res. 1219:1.
- Penta, K. et al. (1999) J. Biol. Chem. 274:11101.
- Rezaee, M. et al. (2002) Am. J. Physiol. Heart Circ. Physiol. 282:H1924.
- Aoka, Y. et al. (2002) Microvasc. Res. 64:148.
- Ho, H.K. et al. (2004) Circulation 109:1314.
- Pollman, M.J. et al. (1999) J. Cell Physiol. 178:359.
- Zhong, J. et al. (2003) J. Clin. Invest. 112:30.
- Kown, M.H. et al. (2003) Ann. Thorac. Surg. 76:1246.
- Choi, E.Y. et al. (2008) Science 322:1101.
Alternate Names
DEL1
Gene Symbol
EDIL3
UniProt
Additional EDIL3 Products
Product Documents for Recombinant Human EDIL3 Protein, CF
Product Specific Notices for Recombinant Human EDIL3 Protein, CF
For research use only
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