Recombinant Human EGF-L6 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8638-EG

R&D Systems, part of Bio-Techne
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8638-EG-050
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Key Product Details

Source

HEK293

Accession #

Q8IUX8

Conjugate

Unconjugated

Applications

Bioactivity

View all EGF-L6 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human EGF-L6 protein
Human EGF-L6
(Asn22-Arg363)
Accession # Q8IUX8		 HP GGGSGGGSGGGS HHHHHH
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn22

Predicted Molecular Mass

40 kDa

SDS-PAGE

40-55 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. Osada, A. et al. (2005) Exp. Cell Res. 303:148.
The ED50 for this effect is 1-5 μg/mL.

Formulation, Preparation and Storage

8638-EG
Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGF-L6

Epidermal Growth Factor-like Domain, Multiple 6 (EGF-L6), also known as MAM- and EGF-containing (MAEG) is a member of the EGF repeat superfamily of proteins. This secreted protein contains five EGF-like repeats, an RGD motif that binds to Integrin alpha8 beta1, and a C-terminal MAM domain (1, 2). Within shared regions, human EGF-L6 shares 75% and 78% amino acid sequence identity with the mouse and rat orthologs, respectively. EGF-L6 is expressed in a multitude of fetal tissues during early development such as lung, heart, liver, spleen, cochlea, and placenta (3, 4). It is also expressed in the dermatome and dermatome-derived tissues including trunk dermis, hair follicles, and cranio-facial mesenchyme (5). EGF-L6 is expressed by osteoblastic-like cells and has been shown to act in a paracrine manner to promote migration of vascular endothelial cells, thus regulating angiogenesis in the local environment of the bone (4, 6). Additionally, EGF-L6 is expressed by epidermal stem cells in the hair follicle bulge and is believed to play a role in regulating the dermal vasculature (7). EGF-L6 is also produced by mature adipocytes and is thought to be involved in the process of adipose tissue expansion and the development of obesity (8). EGF-L6 expression is increased in fibroblastic meningioma and ovarian cancer and has been proposed as a biomarker for the latter (9, 10).

References

  1. Buchner, G. et al. (2000) Genomics 65:16.
  2. Osada, A. et al. (2005) Exp. Cell Res. 303:148.
  3. Yeung, G. et al. (1999) Genomics 62:304.
  4. Chim, S.M. et al. (2011) J. Biol. Chem. 286:22035.
  5. Buchner, G. et al. (2000) Mech. Dev. 98:179.
  6. Chim, S.M. et al. (2013) Cytokine Growth Factor Rev. 24:297.
  7. Xiao, Y. et al. (2013) J. Invest. Dermatol. 133:2324.
  8. Oberauer, R. et al. (2010) Mol. Cell. Biochem. 343:257.
  9. Buckanovich, R.J. et al. (2007) J. Clin. Oncol. 25:852.
  10. Wang, X. et al. (2012) PLoS ONE 7:e52707.

Long Name

EGF-like Domain, Multiple 6

Alternate Names

EGFL6, MAEG

Entrez Gene IDs

25975 (Human); 54156 (Mouse); 317470 (Rat)

Gene Symbol

EGFL6

UniProt

Q8IUX8

Additional EGF-L6 Products

Product Documents for Recombinant Human EGF-L6 Protein, CF

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Product Specific Notices for Recombinant Human EGF-L6 Protein, CF

For research use only

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