Recombinant Human ELFN1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10090-EF

R&D Systems, part of Bio-Techne
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10090-EF-050
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Key Product Details

Source

CHO

Accession #

P0C7U0

Conjugate

Unconjugated

Applications

Bioactivity

View all ELFN1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ELFN1 protein
Asp28-Tyr418, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp28

Predicted Molecular Mass

44 kDa

SDS-PAGE

78-121 kDa, reducing conditions

Activity

Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons.
1.25-2.5 μg/mL of protein is able to significantly inhibit neurite outgrowth.

Scientific Data Images for Recombinant Human ELFN1 His-tag Protein, CF

Recombinant Human ELFN1 His-tag Protein SDS-PAGE

Recombinant Human ELFN1 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human ELFN1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 78-121 kDa.

Formulation, Preparation and Storage

10090-EF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: ELFN1

ELFN1 (Extracellular leucine-rich repeat and fibronectin type-III domain-containing protein 1), also known as Protein phosphatase 1 regulatory subunit 28, is a member of the extracellular Leucine-Rich Repeat superfamily (1). Expressed mainly in the nervous system, ELFN1 is a transmembrane protein that inhibits the activity of protein phosphatase 1 (PP1) complexes (2). Mature human ELFN1 consists of a 391 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment and a 389 aa cytoplasmic tail. The ECD includes one fibronectin type-III domain, six leucine-rich repeats (LRR) and one LRR C-terminal (LRRCT) domain. Human ELFN1 shares 90% aa sequence identity with mouse and rat ELFN1. The cytoplasmic tail contains many tyrosine but no other detectable motifs. ELFN1 is strongly expressed in globus pallidus and interneurons in cortex and hippocampus in both developing and adult brains (1). It is also expressed in endocrine and reproductive tissues (1). Given the functions and discrete patterns of many known LRR family proteins, it has been proposed that ELFN1 could serve as a neuronal adhesion molecule and play an integral role in synapse formation and differentiation via the coordination of both pre- and postsynaptic machineries, thereby involved in neurite outgrowth, axon guidance, fasciculation, and synapse formation (3). Recent studies showed that ELFN1 physically anchor metabotropic glutamate receptor 6 (mGluR6) and mGluR7 across retinal and hippocampal synapses (3-4), and can be recruited selectively to all group III mGluRs (mGluR4, mGluR6, mGluR7, and mGluR8) to allosterically modulate these receptors (5).

References

  1. Dolan J. et al. (2007) BMC Genomics 8:320.
  2. Hendrickx, A. et al. (2009) Chem. Biol. 16:365.
  3. Williams, M.E. et al. (2010) Neuron 68:9.
  4. De Wit, J. and Ghosh A. (2016) Nat Rev Neurosci 17:22.
  5. Dunn H. A. et al. (2018) PNAS 115:5022.

Long Name

Extracellular Leucine-Rich Repeat And Fibronectin Type III Domain Containing 2

Alternate Names

PPP1R28

Entrez Gene IDs

392617 (Human); 243312 (Mouse); 288512 (Rat)

Gene Symbol

ELFN1

UniProt

P0C7U0

Additional ELFN1 Products

Product Documents for Recombinant Human ELFN1 His-tag Protein, CF

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Product Specific Notices for Recombinant Human ELFN1 His-tag Protein, CF

For research use only

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