Recombinant Human Enolase 2 His-tag Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 10412-EN
Key Product Details
Product Specifications
Source
E. coli-derived human Enolase 2/Neuron-specific Enolase protein
Met1-Leu434
with an N-terminal Met and 6-His tag
Met1-Leu434
with an N-terminal Met and 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
48 kDa
SDS-PAGE
46 kDa, under reducing conditions
Activity
Measured by its ability to convert phosphoglyceric acid to phosphoenolpyruvate.
The specific activity is >6,000 pmol/min/μg, as measured under the described conditions.
The specific activity is >6,000 pmol/min/μg, as measured under the described conditions.
Scientific Data Images for Recombinant Human Enolase 2 His-tag Protein, CF
Recombinant Human Enolase 2 His-tag Protein Binding Activity
2 μg/lane of Recombinant Human Enolase 2/Neuron-specific Enolase His-tag (Catalog # 10412-EN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band at 46 kDa under reducing conditions.Formulation, Preparation and Storage
10412-EN
Formulation | Supplied as a 0.2 μm filtered solution in MES, NaCl, KCl and MgSO4. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Enolase 2/Neuron-specific Enolase
References
- Marangos, P.J. and D.E. Schmechel, (1987) Annu. Rev. Neurosci. 10:269.
- Huang, L. et al. (2017) Oncotarget 38:64358.
- Muoio, B. et al. (2018) Int. J. Markers 33:10.
- Yang, H. et al. (2014) PLoS One. 9:e94304.
- Cheng, F. et al. (2014) PLoS One 9:e106680.
- Haque, A. et al. (2016) Metab. Brain Dis. 31:487.
- Anand, N. et al. (2005) Cerebrovasc. Dis. 20:213.
- Mercier, E. et al. (2018) Brain Inj. 32:29.
- Isgro, M.A. et al. (2015) Adv. Exp. Med. Biol. 867:125.
- Hafner, A. et al. (2013) Aging Cell 12:604.
- Polcyn, R. et al. (2017) J. Clin. Cell. Immunol. 8:536.
- Haque, A. et al. (2017) Neurochem. Res. 42:2777.
- Obermajer, N. et al. (2009) Int. J. Biochem. Cell Biol. 41:1685.
- Hafner, A. et al. (2012) Biochem. J. 443:439.
- Leonard, P.G. et al. (2016) Nat. Chem. Bio. 12:1053.
- Muller, F.L. et al. (2012) Nature. 488:337.
Alternate Names
ENO2, gamma-Enolase, Neuronspecific Enolase, NSE
Gene Symbol
ENO2
UniProt
Additional Enolase 2/Neuron-specific Enolase Products
Product Documents for Recombinant Human Enolase 2 His-tag Protein, CF
Product Specific Notices for Recombinant Human Enolase 2 His-tag Protein, CF
For research use only
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