Recombinant Human EphA4 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6827-A4

R&D Systems, part of Bio-Techne
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6827-A4-050
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Key Product Details

Source

NS0

Accession #

NP_004429.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all EphA4 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human EphA4 protein
Human EphA4
(Met1 - Thr547)
Accession # NP_004429.1		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val20

Predicted Molecular Mass

85 kDa (monomer)

SDS-PAGE

93-98 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Human EphA4 Fc Chimera is coated at 2 μg/mL (100 μL/well), the concentration of biotinylayed Recombinant Human Ephrin-A5 Fc Chimera (Catalog # 374-EA) that produces 50% of the optimal binding response is found to be approximately 5-25 ng/mL.

Formulation, Preparation and Storage

6827-A4
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphA4

EphA4, also known as Hek8, Tyro1, and Sek, is a 120 ‑ 130 kDa glycosylated member of the Eph family of transmembrane receptor tyrosine kinases (1, 2). The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. EphA4 is unusual in its ability to be activated by both Ephrin-A and -B molecules, although its interactions with Ephrin-B2 and -B3 are weaker than with Ephrin-A ligands (3, 4). Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. The 528 amino acid (aa) extracellular domain (ECD) of human EphA4 contains an N-terminal Ephrin binding region, a cysteine-rich region, and two fibronectin type III domains (FnIII). The 417 aa cytoplasmic domain contains the tyrosine kinase domain and a sterile alpha motif (SAM) (5). Within the ECD, human EphA4 shares 98% aa sequence identity with mouse and rat EphA4. EphA4 is activated by interactions with Ephrin ligands, triggering a repulsive effect on neurite outgrowth (6, 7). This function is important for the accurate guidance and pathfinding of axons in the spiral ganglion of the cochlea, the anterior commissure, and the corticospinal tract (6 ‑ 8). Neuronal EphA4 interactions with astrocyte‑expressed Ephrins also plays a critical role in long term potentiation by regulating hippocampal neuron dendrite arborization, spine maturation, and function (9 - 11). The up‑regulation of EphA4 in gastric carcinoma is negatively correlated with patient survival (12). In glioma, EphA4 associates with FGF R1, and this enhances FGF basic-induced tumor cell migration (13). EphA4 is also involved in morphogenesis of the thymic epithelium and T cell development (14).

References

  1. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
  2. Merlos-Suarez, A. and E. Batlle (2008) Curr. Opin. Cell Biol. 20:194.
  3. Gale, N.W. et al. (1996) Neuron 17:9.
  4. Qin, H. et al. (2010) J. Biol. Chem. 285:644.
  5. Fox, G.M. et al. (1995) Oncogene 10:897.
  6. Brors, D. et al. (2003) J. Comp. Neurol. 462:90.
  7. Ho, S.K.Y. et al. (2009) Neuroscience 160:784.
  8. Canty, A.J. et al. (2006) Proc. Natl. Acad. Sci. 103:15629.
  9. Murai, K.K. et al. (2003) Nat. Neurosci. 6:153.
  10. Filosa, A. et al. (2009) Nat. Neurosci. 12:1285.
  11. Fu, A.K.Y. et al. (2011) Nat. Neurosci. 14:181.
  12. Oki, M. et al. (2008) World J. Gastroenterol. 14:5650.
  13. Fukai, J. et al. (2008) Mol. Cancer Ther. 7:2768.
  14. Munoz, J.J. et al. (2006) J. Immunol. 177:804.

Long Name

Eph Receptor A4

Alternate Names

Cek8, Hek8, Sek1, Tyro1

Entrez Gene IDs

2043 (Human); 13838 (Mouse)

Gene Symbol

EPHA4

UniProt

NP_004429.1

Additional EphA4 Products

Product Documents for Recombinant Human EphA4 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human EphA4 Fc Chimera Protein, CF

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