Recombinant Human EphA6 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5606-A6

R&D Systems, part of Bio-Techne
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5606-A6-050
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Key Product Details

Source

CHO

Accession #

AAI56734

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

View all EphA6 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human EphA6 protein
Human EphA6
(Trp23-Val550)
Accession # AAI56734		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Trp23

Predicted Molecular Mass

85.7 kDa (monomer)

SDS-PAGE

95-110 kDa under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When rhEphA6/Fc Chimera is immobilized at 2 μg/mL, 100 μL/well, it binds biotinylated rhEphrin-A3/Fc Chimera with a linear range of
0.31-20 ng/mL.

Formulation, Preparation and Storage

5606-A6
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphA6

EphA6, also known as Ehk2 and Hek12, is a 110 kDa member of the transmembrane Eph receptor tyrosine kinase family. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1 - 3). The 527 amino acid (aa) extracellular domain (ECD) of human EphA6 contains an N-terminal Ephrin binding region, a cysteine-rich region, and two fibronectin type II domains. The 465 aa cytoplasmic domain contains the tyrosine kinase domain and a sterile alpha motif (SAM) (4, 5). Alternate splicing generates an isoform that consists of the transmembrane segment and a portion of the kinase domain. Within the ECD, human EphA6 shares 95% aa sequence identity with mouse and rat EphA6. EphA6 binds and is activated by all five of the Ephrin-A ligands (6). It is primarily expressed in the brain and testes with lesser amounts in the colon and inner ear (4, 5, 7). Within the developing brain, it is localized to deep layers of the posterior cortical plate (8, 9). In adult rodents, EphA6 is expressed on Purkinje cells, in the accessory olfactory bulb, and on vestibular ganglion neurons of the inner ear (10, 11). This pattern is complementary to the expression of its ligand, Ephrin-A5 (9, 11). EphA6 null mice exhibit defects in learning and memory formation (12). EphA6 expression in vascular endothelium of the genital tubercle is under the regulation of HOX13, and a lack of HOX13 leads to hypospadias in mice (13). EphA6 is downregulated in human colon and renal cancers (7).

References

  1. Pasquale, E.B. (2008) Cell 133:38.
  2. Merlos-Suarez, A. and E. Batlle (2008) Curr. Opin. Cell Biol. 20:194.
  3. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
  4. Lee, A.M. et al. (1996) DNA Cell Biol. 15:817.
  5. Maisonpierre, P.C. et al. (1993) Oncogene 8:3277.
  6. Gale, N.W. et al. (1996) Neuron 17:9.
  7. Hafner, C. et al. (2004) Clin. Chem. 50:490.
  8. Donoghue, M.J. and P. Rakic (1999) J. Neurosci. 19:5967.
  9. Yun, M.E. et al. (2003) J. Comp. Neurol. 456:203.
  10. Matsunaga, T. et al. (2000) Eur. J. Neurosci. 12:1599.
  11. Knoll, B. et al. (2001) Development 128:895.
  12. Savelieva, K.V. et al. (2008) Neurosci. Lett. 438:205.
  13. Shaut, C.A. et al. (2007) Dev. Dyn. 236:951.

Long Name

Eph Receptor A6

Alternate Names

Ehk2, Hek12

Entrez Gene IDs

285220 (Human); 13840 (Mouse)

Gene Symbol

EPHA6

UniProt

AAI56734

Additional EphA6 Products

Product Documents for Recombinant Human EphA6 Fc Chimera Protein, CF

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