Recombinant Human Ephrin-A2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7856-A2

R&D Systems, part of Bio-Techne
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7856-A2-050
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Key Product Details

Source

NS0

Accession #

O43921

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Ephrin-A2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Ephrin-A2 protein
Human Ephrin-A2
(Glu27-Asn188)
Accession # O43921		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Starts at Glu27

Predicted Molecular Mass

45.1 kDa (monomer)

SDS-PAGE

55-60 kDa, reducing conditions

Activity

Measured by its ability to inhibit proliferation of PC-3 human prostate cancer cells.
The ED50 for this effect is 5-25 ng/mL.

Formulation, Preparation and Storage

7856-A2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ephrin-A2

Ephrin‑A2, also known as ELF‑1, HEK7‑L, LERK‑6, and EPLG6, is an approximately 20 kDa member of the GPI‑linked Ephrin‑A family of proteins that bind and induce the tyrosine autophosphorylation of Eph receptors. In particular, Ephrin‑A2 preferentially interacts with receptors of the EphA family of proteins. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, axon guidance and cancer progression. (1‑3). Human Ephrin‑A2 is synthesized as a 213 amino acid (aa) preproprecursor that contains a 24 aa signal peptide, a 164 aa mature chain, and a 25 aa C‑terminal propeptide that is removed prior to GPI linkage of Ephrin‑A2 to the membrane (4, 5). The mature region is structurally related to the extracellular domains of the transmembrane Ephrin‑B ligands (1, 3), and shares 93% aa sequence identity with mouse and rat Ephrin‑A2. Ephrin‑A2 is expressed in discrete regions of the developing nervous system and limb buds (6‑9). Its distribution complements the pattern of Eph receptor expression, and this plays an important role in tissue morphogenesis (9‑11). Ephrin‑A2 exerts an axon repulsive signal which is important for the accurate pathfinding of retinal ganglion cell axons to the tectum and hippocampal axons to the lateral septum (10, 12). Its up‑regulation on astrocytes at sites of optic nerve damage may prevent re‑innervation by retinal ganglion cells (13). Ephrin‑A2 is also expressed on neural progenitor cells in the subventricular zone (SVZ). It interacts with EphA7, triggering reverse signaling through Ephrin‑A2 and inhibition of progenitor cell proliferation (10). In the developing limbs, Ephrin‑A2 regulates cartilage morphogenesis and the projection of motoneuron axons (8, 9, 14).

References

  1. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  2. Astin, J.W. et al. (2010) Nat. Cell Biol. 12:1194.
  3. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  4. Aasheim, H.-C. et al. (1998) Biochem. Biophys. Res. Commun. 252:378.
  5. Cerretti, D.P. and N. Nelson (1998) Genomics 47:131.
  6. Cheng, H.-J. et al. (1994) Cell 79:157.
  7. Kenmuir, C.L. et al. (2012) Anat. Rec. (Hoboken) 295:105.
  8. Ohta, K. et al. (1997) Mech. Dev. 64:127.
  9. Wada, N. et al. (2003) Dev. Biol. 264:550.
  10. Gao, P.-P. et al. (1996) Proc. Natl. Acad. Sci. USA 93:11161.
  11. Holmberg, J. et al. (2005) Genes Dev. 19:462.
  12. Nakamoto, M. et al. (1996) Cell 86:755.
  13. Symonds, A.C.E. et al. (2007) Eur. J. Neurosci. 25:744.
  14. Eberhart, J. et al. (2000) Dev. Neurosci. 22:237.

Alternate Names

Cek7-L, EFNA2, ELF-1, EphrinA2, LERK6

Entrez Gene IDs

1943 (Human); 13637 (Mouse)

Gene Symbol

EFNA2

UniProt

O43921

Additional Ephrin-A2 Products

Product Documents for Recombinant Human Ephrin-A2 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human Ephrin-A2 Fc Chimera Protein, CF

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