Recombinant Human Ephrin-B3 Fc Chimera Biotinylated Protein

R&D Systems, part of Bio-Techne | Catalog # BT395

R&D Systems, part of Bio-Techne
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Q15768

Structure / Form

Disulfide-linked homodimer

Conjugate

Biotin

Applications

Binding Activity

View all Ephrin-B3 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Ephrin-B3 protein
Human Ephrin-B3
(Leu28-Ser224)
Accession # Q15768		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

N-terminal Sequence Analysis

Leu28

Predicted Molecular Mass

49.2 kDa (monomer)

SDS-PAGE

58 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized recombinant mouse EphB3 Fc Chimera at 2 µg/mL (100 µL/well) can bind biotinylated Recombinant Human Ephrin‑B3 Fc Chimera with a linear range of 1.3-80 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

BT395
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution

Reconstitute at 100 μg/mL with sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ephrin-B3

Ephrin-B3, also known as Elk-L3, LERK8, Eplg8, NLERK-2, and EFL6, is an approximately 50 kDa member of the Ephrin-B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains (ECD) of Ephrin-B ligands are structurally related to GPI-anchored Ephrin-A ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-B3 preferentially interacts with receptors in the EphB family and also with EphA4. The binding of Ephrin-B3 to Eph proteins also triggers reverse signaling through Ephrin-B3 (1, 2). Mature human Ephrin-B3 consists of a 199 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 93 aa cytoplasmic domain (3, 4). Within the ECD, human Ephrin-B3 shares 96% and 97% aa sequence identity with mouse and rat Ephrin-B3, respectively. Ephrin-B3 is expressed on oligodendrocytes and neurons in the hippocampus and along the midline of the spinal cord (5-9). It is up-regulated in glioma and promotes tumor cell invasion and migration (10). Ephrin-B3 functions as a repulsive axon guidance molecule by inducing growth cone collapse, neurite retraction, and axon pruning (5-8). Its repulsive effect along the spinal cord midline restricts motor neuron axons to their ipsilateral sides, thereby maintaining the independence of voluntary left side/right side movements (8, 9). Ephrin-B3 plays a role in the regulation of excitatory synapse density and synaptic maturation (6, 11, 12). It also functions as a cellular receptor for Nipah virus (13) and can induce the migration of memory B cells (14).

References

  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Gale, N.W. et al. (1996) Oncogene 13:1343.
  4. Nicola, N.A. et al. (1996) Growth Factors 13:141.
  5. Benson, M.D. et al. (2005) Proc. Natl. Acad. Sci. USA 102:10694.
  6. Xu, N.-J. et al. (2011) Nat. Neurosci. 14:1421.
  7. Xu, N.-J. and M. Henkemeyer (2009) Nat. Neurosci. 12:268.
  8. Kullander, K. et al. (2001) Genes Dev. 15:877.
  9. Yokoyama, N. et al. (2001) Neuron 29:85.
  10. Nakada, M. et al. (2006) Cancer Res. 66:8492.
  11. McClelland, A.C. et al. (2010) Proc. Natl. Acad. Sci. USA 107:8830.
  12. Antion, M.D. et al. (2010) Mol. Cell. Neurosci. 45:378.
  13. Negrete, O.A. et al. (2006) PLoS Pathog. 2:e7.
  14. Holen, H.L. et al. (2011) Scand. J. Immunol. 74:144.

Alternate Names

EFL-6, Efnb3, Elk-L3, EphrinB3, Epl8, LERK-8, NLERK-2

Entrez Gene IDs

1949 (Human); 13643 (Mouse)

Gene Symbol

EFNB3

UniProt

Q15768

Additional Ephrin-B3 Products

Product Documents for Recombinant Human Ephrin-B3 Fc Chimera Biotinylated Protein

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Product Specific Notices for Recombinant Human Ephrin-B3 Fc Chimera Biotinylated Protein

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