Recombinant Human ErbB3/Her3 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10368-RB

R&D Systems, part of Bio-Techne
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10368-RB-050
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Key Product Details

Source

HEK293

Accession #

P21860.1

Conjugate

Unconjugated

Applications

Bioactivity

View all ErbB3/Her3 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human ErbB3/Her3 protein
Human ErbB3/Her3
(Ser20-Gly538)
Accession # P21860-1		 YSKGSQSRMGGGGALQWNCSGGIQ  6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser20

Predicted Molecular Mass

61 kDa

SDS-PAGE

73-83 kDa, under reducing conditions

Activity

Measured by its ability to inhibit the biological activity of Neuregulin-1-beta 1 on MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.
The ED50 for this effect is 0.3-3 µg/mL in the presence of 10 ng/mL Recombinant Human NRG1‑ beta1/HRG1‑ beta1 Extracellular Domain (Catalog # 377-HB).

Scientific Data Images for Recombinant Human ErbB3/Her3 His-tag Protein, CF

Recombinant Human ErbB3/Her3 His-tag Protein Bioactivity

Recombinant Human ErbB3/Her3 His-tag Protein Bioactivity

Recombinant Human ErbB3/Her3 His-tag (Catalog # 10368-RB) inhibits the biological activity of Recombinant Human NRG1-beta 1/HRG1-beta 1 ECD Protein (Catalog # 377-HB) on MCF-7 human breast cancer cells. The ED50 for this effect is 0.3-3 µg/mL.
Recombinant Human ErbB3/Her3 His-tag Protein SDS-PAGE

Recombinant Human ErbB3/Her3 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human ErbB3/Her3 His-tag (Catalog # 10368-RB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 73-83 kDa.

Formulation, Preparation and Storage

10368-RB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ErbB3/Her3

ErbB3, also called Her3 (human epidermal growth factor receptor 3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. Among ErbB family members, ErbB3 is unique in that it contains a defective kinase domain. ErbB3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric ErbB3 serves as a low affinity receptor for the heregulins (HRG). ErbB3 heterodimerizes with ErbB2 to form a high affinity receptor complex. In contrast, ErbB3 homodimerization or heterodimerization with ErbB4 forms a low affinity heregulin-binding complex. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. The cytoplasmic domain of ErbB3 also contains six consensus binding motifs for the SH2 domain of the regulatory p85 subunit of phosphoinositide 3-kinase (PI 3-kinase, PI3K) as well as one proline-rich consensus binding motif for the SH3 domain of p85. Human ErbB3 consists of 1342 amino acids (aa) with a 19 aa signal sequence, a 624 aa extracellular domain, a 21 aa transmembrane region, and a 678 aa cytoplasmic domain. ErbB3 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation.

References

  1. Kraus, M.H. et al. (1989) Proc. Natl. Acad. Sci. 86:9193.
  2. Plowman, G.D. et al. (1990) Proc. Natl. Acad. Sci. USA 87:4905.
  3. Carraway, K.L. 3rd et al. (1994) J. Biol. Chem. 269:14303.
  4. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
  5. Sundaresan, S. et al. (1998) Endocrinology 139:4756.
  6. Hellyer, N.J. et al. (1998) Biochem. J. 333:757.
  7. Schaefer, G. et al. (1999) J. Biol. Chem. 274:859.
  8. Hellyer, N.J. et al. (2001) J. Biol. Chem. 276:42153.
  9. Schlessinger, J. (2000) Cell 103:211.
  10. Daly, R.J. (1999) Growth Factors 16:255.

Long Name

Receptor Tyrosine Protein Kinase ErbB3

Alternate Names

HER3

Entrez Gene IDs

2065 (Human); 13867 (Mouse)

Gene Symbol

ERBB3

UniProt

P21860.1

Additional ErbB3/Her3 Products

Product Documents for Recombinant Human ErbB3/Her3 His-tag Protein, CF

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Product Specific Notices for Recombinant Human ErbB3/Her3 His-tag Protein, CF

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