Recombinant Human FCAR/CD89 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3939-FA

R&D Systems, part of Bio-Techne
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3939-FA-050
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Key Product Details

Source

NS0

Accession #

P24071

Conjugate

Unconjugated

Applications

Binding Activity

View all FCAR/CD89 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human FCAR/CD89 protein
Gln22-Asn227, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln22 predicted

Predicted Molecular Mass

24.3 kDa

SDS-PAGE

40-50 kDa, reducing conditions

Activity

Measured by its ability to bind human lgA in a functional ELISA with an estimated
KD <10 nM.

Formulation, Preparation and Storage

3939-FA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCAR/CD89

FCAR, also called Fc alphaRI or CD89, is a variably glycosylated 50-100 kDa myeloid-specific type I transmembrane (TM) Fc receptor for IgA that is a member of the multichain immune recognition receptor (MIRR) family (1-3). Human FCAR contains a 21 amino acid (aa) signal sequence and extracellular (ECD), TM and cytoplasmic domains of 206, 19 and 41 aa, respectively (4). Arg230 within the TM domain supports interaction with the ITAM-containing signaling subunit, FcR gamma, which contains a TM Asp (5-7). Two ECD C2-type Ig-like domains (EC1 and 2) are oriented at right angles (8). Up to two molecules of FCAR can bind one molecule of serum IgA via EC1 (8). Many splice variants have been reported, but only two have been identified as proteins (9, 10). The a.2 form, which lacks 22 aa just prior to the TM domain, is exclusively expressed in alveolar macrophages. The a.3 form lacks EC2. FCAR binds monomeric, polymeric and secretory IgA, but does not mediate the barrier function of secretory IgA in mucosal epithelium (1-3). Shedding and circulation of polymeric IgA/FCAR immune complexes has been reported (11). Circulating neutrophils, eosinophils, and monocytes express FCAR (12). Tissue expression of FCAR is mainly from neutrophils; FCAR is downregulated as monocytes differentiate to tissue macrophages (12). On neutrophils, a significant amount of FCAR lacks FcR gamma, but can still be endocytosed to early endosomes and recycled to the cell surface (5). Binding of serum IgA to FCAR is transient and anti-inflammatory, inhibiting IgG or IgE-induced degranulation (6). Sustained aggregation of FCAR results in inflammatory responses (6). FcR gamma signaling is required for these and for transport to late endosomes (5-7). Human FCAR shows 55-58% aa identity with rat, horse and cow FCAR. No ortholog occurs in mouse. FCAR structure resembles the KIR/ILT/LIR/MIR family more than other IgA receptors, including pIgR, Fc alpha/μR, asialoglycoprotein receptor (ASGR1) and transferrin receptor (TfR) (1-3).

References

  1. Wines, B. D. and P. M. Hogarth (2006) Tissue Antigens 68:103.
  2. Otten, M. A. and M. van Egmon (2004) Immunol. Lett. 92:23.
  3. Montiero, R. C. and J. G. J. van de Winkel (2003) Annu. Rev. Immunol. 21:177.
  4. Maliszewski, C. R. et al. (1990) J. Exp. Med. 172:1665.
  5. Launay, P. et al. (1999) J. Biol. Chem. 274:7216.
  6. Pasquier, B. et al. (2005) Immunity 22:31.
  7. Shen, L. et al. (2001) Blood 97:205.
  8. Herr, Y. et al. (2003) Nature 423:614.
  9. Patry, C. et al. (1996) J. Immunol. 156:4442.
  10. Togo, S. et al. (2003) FEBS Lett. 535:205.
  11. van der Boog, P. J. M. et al. (2002) J. Immunol. 168:1252.
  12. Hamre, R. et al. (2003) Scand. J. Immunol. 57:506.

Long Name

IgA Fc Receptor

Alternate Names

CD89

Entrez Gene IDs

2204 (Human); 365183 (Rat); 102145896 (Cynomolgus Monkey)

Gene Symbol

FCAR

UniProt

P24071

Additional FCAR/CD89 Products

Product Documents for Recombinant Human FCAR/CD89 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human FCAR/CD89 Protein, CF

For research use only

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