Recombinant Human FCRLB/FCRY Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4705-FC

R&D Systems, part of Bio-Techne
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4705-FC-050
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Key Product Details

Source

NS0

Accession #

Q6BAA4

Conjugate

Unconjugated

Applications

Bioactivity

View all FCRLB/FCRY Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human FCRLB/FCRY protein
Ala18-Ser426 with a C-terminal 6x His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala18

 

Predicted Molecular Mass

46 kDa

 

SDS-PAGE

65-70 kDa, reducing conditions

Activity

Measured by its ability to bind human IgG with an estimated KD < 120 nM.

Formulation, Preparation and Storage

4705-FC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRLB/FCRY

FCRLB, also known as FCRL2, FCRY, and FREB2, is a 60 kDa protein with sequence homology to classical Fc receptors. There are at least six type 1 transmembrane FCRL proteins and two that lack transmembrane segments. Each family member contains between three and nine immunoglobulin-like domains. FCRL proteins are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRLB binds to purified human IgG with high affinity. Human FCRLB shares 82% and 85% amino acid sequence identity with mouse and rat FCRLB, respectively. It contains a putative signal peptide, three immunoglobulin-like domains, and a mucin-like stalk that is rich in Pro, Ser, and Thr residues (1-3). The stalk region also contains di-Leu motifs and an unpaired cysteine (1-3). Alternative splicing generates isoforms with deletions in the putative signal peptide and substitutions and/or truncations in the third Ig-like domain (3). When expressed in transfectants, FCRLB is not secreted but shows a diffuse intracellular localization (3, 4). FCRLB is expressed at a low level in placenta and in B lineage cells of the germinal center (3, 4). It is up-regulated in B cells by BAFF and LPS (5). Among non-hematopoietic cells, FCRLB is expressed in fibroblasts, melanocytes, and melanoma (3, 5). It is preferentially expressed in nonproliferating cells and at the onset of apoptosis (4, 5).

References

  1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
  2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
  3. Chikaev, N.A. et al. (2005) Genomics 85:264.
  4. Wilson, T.J. and M. Colonna (2005) Genes Immun. 6:341.
  5. Masuda, K. et al. (2005) Gene 363:32.

Long Name

Fc Receptor-like B

Alternate Names

FcRL2, FCRLM2, FCRLY, FCRY, FREB-2

Entrez Gene IDs

127943 (Human); 435653 (Mouse); 498275 (Rat)

Gene Symbol

FCRLB

UniProt

Q6BAA4 (Human)

Additional FCRLB/FCRY Products

Product Documents for Recombinant Human FCRLB/FCRY Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human FCRLB/FCRY Protein, CF

For research use only

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