Recombinant Human Fetuin A/AHSG Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1184-PI

R&D Systems, part of Bio-Techne
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1184-PI-050
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Key Product Details

Source

NS0

Accession #

P02765

Conjugate

Unconjugated

Applications

Inhibition Activity

View all Fetuin A/AHSG Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Fetuin A/AHSG protein
Ala19-Val367, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala19

Predicted Molecular Mass

39 kDa

SDS-PAGE

58 kDa, reducing conditions

Activity

Measured by its ability to inhibit calcium phosphate precipitation. Schinke, T. et al. (1996) J. Biol. Chem. 271:20789.
The IC50 value is <50 μg/mL, as measured under the described conditions.

Formulation, Preparation and Storage

1184-PI
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 500 μg/mL in sterile 25 mM Tris and 150 mM NaCl, pH 7.5.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fetuin A/AHSG

Human Fetuin A, also known as alpha2-Heremans-Schmid glycoprotein, is encoded by the AHSG gene. It is a major plasma protein and a member of the cystatin superfamily of protease inhibitors (1, 2). It is expressed by hepatocytes, the principal cell source, and by monocyte/macrophages (3). The major form of plasma Fetuin A corresponds to a disulfide bond-linked two chains derived from the single chain (4). Human Fetuin A has a number of functions. It is a negative acute-phase protein with normal circulating levels in adults (300‑600 μg/mL), which fall significantly (30‑50%) during injury and infection (5). It enhances entry of cationic inhibitors into macrophages (6). It inhibits both insulin receptor autophosphorylation and undesirable calcification (6, 7). The purified recombinant human Fetuin A corresponds to the single chain, which can be converted to the two-chain form by recombinant human Furin (Catalog # http://www.rndsystems.com/product_results.aspx?k=1503-SE">1503-SE) in vitro. However, the conversion does not enhance its inhibitory activity against recombinant human Cathpesin V, a cysteine protease.

References

  1. Kellemann, J. et al. (1989) J. Biol. Chem. 264:14121.
  2. Dziegielewska, K.M. et al. (1990) J. Biol. Chem. 265:4354.
  3. Dziegielewska, K.M. et al. (1996) Histochem. Cell Biol. 106:319.
  4. Gejyo, F. and K. Schmid (1981) Biochim. Biophys. Acta. 671:78.
  5. Wang, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14429.
  6. Mathews, S.T. et al. (2000) Mol. Cell Endocrinol. 164:87.
  7. Schäfer, C. et al. (2003) J. Clin. Invest. 112:357.

Long Name

alpha-2-HS Glycoprotein

Alternate Names

AHSG, alpha-2-HS-glycoprotein

Entrez Gene IDs

197 (Human); 11625 (Mouse); 25373 (Rat)

Gene Symbol

AHSG

UniProt

P02765

Additional Fetuin A/AHSG Products

Product Documents for Recombinant Human Fetuin A/AHSG Protein, CF

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Product Specific Notices for Recombinant Human Fetuin A/AHSG Protein, CF

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