Recombinant Human FGFR2 alpha (IIIc) Fc Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI712

Fc Chimera Biotinylated
R&D Systems, part of Bio-Techne
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AVI712-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

P21802.1

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all FGFR2 alpha Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human FGFR2 alpha protein
Human FGF R2 alpha (IIIc)
(Arg22-Glu377)
Accession # P21802.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg22

Predicted Molecular Mass

68 kDa (monomer)

SDS-PAGE

90-110 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
In a Human FGF acidic/FGF1 antibody (Catalog # AF232) coated plate, in the presence of 50.0 ng/mL of Recombinant Human FGF acidic/FGF1  (Catalog # 232-FA), Biotinylated Recombinant Human FGFR2 alpha (IIIc) Fc Chimera Avi-tag Protein binds with an ED50 of 0.0750-0.450 µg/mL.

Scientific Data Images for Recombinant Human FGFR2 alpha (IIIc) Fc Avi-tag Protein, CF

Biotinylated Recombinant Human FGFR2 alpha (IIIc) Fc Chimera Avi-tag Protein Binding Activity.

In a Human FGF acidic/FGF1 antibody (AF232) coated plate, in the presence of 50.0 ng/mL of Recombinant Human FGF acidic/FGF1 (232-FA), Biotinylated Recombinant Human FGFR2 alpha (IIIc) Fc Avi-tag Protein (Catalog # AVI712) binds with an ED50 of 0.0750-0.450 µg/mL.

Biotinylated Recombinant Human FGFR2 alpha (IIIc) Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human FGFR2 alpha (IIIc) Fc Chimera Avi-tag Protein (Catalog # AVI712) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 90-110 kDa and 180-220 kDa, respectively.

Formulation, Preparation and Storage

AVI712
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR2 alpha

Fibroblast growth factor receptor 2 (FGFR2) belongs to a family of type I transmembrane tyrosine kinases which mediate the biological functions of FGFs that are involved in a multitude of physiological and pathological cellular processes (1). The FGFR family is comprised of 4 structurally conserved members (FGFR1-4) all possessing and extracellular domain (ECD) with three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain (1, 2). The ECD of mature, full-length FGFR2 shares 95% amino acid sequence identity with mouse FGFR2. Alternative splicing generates multiple forms of FGFR1-3, each with unique signaling characteristics (1-3). For FGFR2, alternative splicing of the ECD, specifically the IgIII domain, results in IIIb, or IIIc isoforms (4). The FGFR splice variants also exhibit distinct and varying binding affinities for different FGF ligands (2,4). Specifically, FGFR2A(IIIc) binds most FGF ligands but not the FGF10 subfamily, while FGFR2A(IIIc) binds only members of the FGF10 subfamily (5). FGFRs mediate the FGF signaling cascade which regulate developmental processes including cellular proliferation, differentiation, and migration, morphogenesis, and patterning (6). FGFRs transduce the signals through three dominant pathways including RAS/MAPK, PI3k/AKT, and PLC gamma (7). While FGFR2 is widely expressed in many adult human tissues, isoform expression is tissue specific, with IIIb predominantly expressed in epithelial cells, while IIIc is expressed in mesenchymal cells (5). FGFR2 signaling is critical for embryonic development, tissue repair, and regulation of osteoblast function and bone growth (8). Mutations in FGFR2 or misregulation of FGFR2 mediated signaling is found in multiple skeletal dysplasias, with FGFR2A(IIIc) specifically upregulated in several cancers including prostate, breast and pancreatic and is proposed as a novel therapeutic target for colorectal carcinomas (6, 9). Our Avi-tag Biotinylated FGFR2A(IIIc) features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Ornitz, D.M. and Itoh, N. (2015) Wiley Interdiscip Rev Dev Biol. 4:215.
  2. Zhang, X. et al. (2006) J Biol Chem. 281:15694.
  3. Ferguson, H.R. et al. (2021) Signaling. Cells 10:1201.
  4. Holzmann, K. et al. (2012) J Nucleic Acids. 2012:950508.
  5. Wagner, E.J. et al. (2003) RNA 9:1552.
  6. Xie, Y. et al. (2020) Sig Transduct Target Ther 5:181.
  7. Mossahebi-Mohammadi, M. et al. (2020) Front Cell Dev Biol. 18:79.
  8. Teven, C.M. et al. (2014) Genes Dis. 1:199.
  9. Matsuda, Y. et al. (2012) Mol Cancer Ther. 11:2010.

Long Name

Fibroblast Growth Factor Receptor 2 alpha

Alternate Names

FGF R2a

Entrez Gene IDs

2263 (Human); 14183 (Mouse)

Gene Symbol

FGFR2

UniProt

P21802.1

Additional FGFR2 alpha Products

Product Documents for Recombinant Human FGFR2 alpha (IIIc) Fc Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human FGFR2 alpha (IIIc) Fc Avi-tag Protein, CF

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