Recombinant Human Fibronectin/Anastellin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8258-FN

R&D Systems, part of Bio-Techne
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8258-FN-050
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Key Product Details

Source

E. coli

Accession #

P02751

Conjugate

Unconjugated

Applications

Bioactivity

View all Fibronectin/Anastellin Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Fibronectin/Anastellin protein
Asn631-Pro705, with an N-terminal Met and a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met & Asn631

Predicted Molecular Mass

9 kDa

SDS-PAGE

9 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to enhance Fibronectin-mediated adhesion of NIH-3T3 mouse embryonic fibroblast cells.
The ED50 for this effect is 0.3-1.5 μg/mL.

Formulation, Preparation and Storage

8258-FN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fibronectin/Anastellin

Fibronectin (FN) is a large modular glycoprotein that is found as a polymeric fibrillar network in the extracellular matrix (ECM) and as soluble disulfide-linked dimeric protomers in plasma and other body fluids (1, 2). Fibronectin is a ligand for many molecules including fibrin, heparin, chondroitin sulfate, collagen/gelatin, and integrins. It is involved in multiple cellular processes such as cell adhesion/migration, blood clotting, morphogenesis, tissue repair, and cell signaling. Fibronectin functions are mediated by the insoluble polymeric fibrils. Conversion of soluble fibronectin to fibronectin fibrils in the ECM is initiated by binding to cell surface integrins, resulting in exposure of cryptic epitopes necessary for polymerization (1). Fibronectin is made up of three types of homologous structural motifs termed FN type I, type II, and type III repeats (3-5). Alternative splicing generates multiple isoforms of fibronectin which may have insertions of extra type III domains (EDA and EDB) or alteration of the type III connecting segment (IIICS) (5). Differential splicing within the IIICS domain determines the presence of CS1 and CS2 sequences and the sensitivity to proteases (6, 7). A fragment from the first type III repeat, known as Anastellin, binds to fibronectin and induces the formation of high molecular weight disulfide-linked superfibronectin (sFN) (8-10). sFN exhibits increased protease sensitivity and cellular adhesiveness compared to fibronectin (8, 10). Anastellin acts in vivo to inhbit angiogenesis, tumor growth, and metastasis (9).

References

  1. Mao, Y. and Schwarzbauer, J.E. (2005) Matrix Biol. 24:389.
  2. Potts, J.R. and Campbell, I.D. (1996) Matrix Biol. 15:313.
  3. Bernard, M.P. et al. (1985) Biochemistry 24:2698.
  4. Kornblihtt, A.R. et al. (1983) Proc. Natl. Acad. Sci. USA 80:3218.
  5. Kornblihtt, A.R. et al. (1985) EMBO J. 4:1755.
  6. Mould, A.P. et al. (1991) J. Biol. Chem. 266:3579.
  7. Abe, Y. et al. (2005) Biochem. Biophys. Res. Commun. 338:1640.
  8. Morla, A. et al. (1994) Nature 367:193.
  9. Yi, M. and E. Rouslahti (2001) Proc. Natl. Acad. Sci. USA 98:620.
  10. Ohashi, T. and H.P. Erickson (2005) J. Biol. Chem. 280:39143.

Alternate Names

CIGDKFZp686O13149, Cold-insoluble globulin, DKFZp686F10164, DKFZp686H0342, DKFZp686I1370, fibronectin, fibronectin 1, FNFINC, FNZ, GFND2ED-B, LETSGFND, migration-stimulating factor, MSF

Entrez Gene IDs

2335 (Human)

Gene Symbol

FN1

UniProt

P02751

Additional Fibronectin/Anastellin Products

Product Documents for Recombinant Human Fibronectin/Anastellin Protein, CF

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Product Specific Notices for Recombinant Human Fibronectin/Anastellin Protein, CF

For research use only

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