Recombinant Human FOLR2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5697-FR

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
5697-FR-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

CHO

Accession #

P14207

Conjugate

Unconjugated

Applications

Binding Activity

View all FOLR2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human FOLR2 protein
Gln22-His228, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln22 predicted

Predicted Molecular Mass

25.4 kDa

SDS-PAGE

25-40 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Folic Acid-Bovine Serum Albumin was coated at 5 μg/mL (100 μL/well), the concentration of Recombinant Human FOLR-2 that produces 50% of the optimal binding response was found to be <1 nM.

Formulation, Preparation and Storage

5697-FR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FOLR2

Folate Receptor 2 (FOLR2), also known as Folate Receptor beta, is a 38 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR2 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3, 4). Human FOLR2 shares 83% amino acid sequence identity with mouse and rat FOLR2. FOLR2 is predominantly expressed in placenta, cells of the neutrophilic lineage, and some CD34+ hematopoietic progenitor cells (4 - 6). It is upregulated on myeloid  leukemias, head and neck squamous cell carcinomas, and several nonepithelial cancers (3, 5, 7). It is also upregulated on macrophages and monocytes at chronic inflammatory sites including rheumatoid arthritis synovium and glioblastoma (8 - 10). FOLR2 knockout mice do not show gross morphological defects, but they exhibit increased sensitivity to arsenate-induced teratogenicity (11, 12).

References

  1. Fowler, B. et al. (2001) Kidney Int. 59:S-221.
  2. Kelemen, L.E. (2006) Int. J. Cancer 119:243.
  3. Shen, F. et al. (1994) Biochemistry 33:1209.
  4. Ratnam, M. et al. (1989) Biochemistry 28:8249.
  5. Ross, J.F. et al. (1999) Cancer 85:348.
  6. Reddy, J.A. et al. (1999) Blood 93:3940.
  7. Ross, J.F. et al. (1994) Cancer 73:2432.
  8. Nakashima-Matsushita, N. et al. (1999) Arthritis Rheum. 42:1609.
  9. van der Heijden, J.W. et al. (2009) Arthritis Rheum. 60:12.
  10. Nagai, T. et al. (2009) Cancer Immunol. Immunother. Feb 24 epub.
  11. Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
  12. Wlodarczyk, B. et al. (2001) Toxicol. Appl. Pharmacol. 177:238.

Long Name

Folate Receptor 2

Alternate Names

beta-HFR, FR-beta, FR-P3

Entrez Gene IDs

2350 (Human); 14276 (Mouse); 293154 (Rat)

Gene Symbol

FOLR2

UniProt

P14207 (Human)

Additional FOLR2 Products

Product Documents for Recombinant Human FOLR2 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human FOLR2 Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.