Recombinant Human FOLR3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5319-FR

R&D Systems, part of Bio-Techne
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5319-FR-050
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Key Product Details

Source

NS0

Accession #

NP_000795

Conjugate

Unconjugated

Applications

Binding Activity

View all FOLR3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human FOLR3 protein
Ala22-Ser245 & Ser26-Ser245 both with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala22 & Ser26

Predicted Molecular Mass

26 kDa

SDS-PAGE

30-40 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Folic Acid-Bovine Serum Albumin is coated at 5 μg/mL (100 μL/well), the concentration of rhFOLR-3 that produces 50% of the optimal binding response is found to be approximately 0.2 -1 nM.

Formulation, Preparation and Storage

5319-FR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FOLR3

Folate receptor 3 (FOLR3), also known as folate receptor gamma, is a 32 kDa protein that binds folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1). Mature FOLR3 is a glycosylated protein that contains an imperfect carboxy terminal GPI anchor sequence (2, 3). A fraction of FOLR3 is GPI-linked to the cell surface, but the majority is released as a secreted protein (2). Mature human FOLR3 shares 74% and 83% amino acid (aa) sequence identity with FOLR1 and FOLR2, respectively. Orthologs of human FOLR3 have not been described in mouse or rat. A genetic polymorphism generates a truncated isoform that lacks the carboxy terminal 139 aa (2, 4). FOLR3 is expressed by cells of the myelocyte and B lymphocyte lineage as well as by various carcinomas (2, 3). FOLR3 is more highly expressed in ovarian carcinomas than in breast carcinomas or mesotheliomas (5). Unlike other folate receptors, FOLR3 does not exhibit stereoselectivity in the binding of folates (3).

References

  1. Fowler, B. et al. (2001) Kidney Int. 59:S-221.
  2. Shen, F. et al. (1994) Biochemistry 33:1209.
  3. Shen, F. et al. (1995) Biochemistry 34:5660.
  4. Wang, H. et al. (1998) Nucl. Acids Res. 26:2132.
  5. Yuan, Y. et al. (2009) Hum. Pathol. May 18 epub.

Long Name

Folate Receptor 3

Alternate Names

FR-gamma

Entrez Gene IDs

2352 (Human)

Gene Symbol

FOLR3

UniProt

NP_000795

Additional FOLR3 Products

Product Documents for Recombinant Human FOLR3 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human FOLR3 Protein, CF

For research use only

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