Recombinant Human Frizzled-9 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9175-FZ

R&D Systems, part of Bio-Techne
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9175-FZ-050
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Key Product Details

Source

HEK293

Accession #

O00144

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Frizzled-9 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Frizzled-9 protein
Human Frizzled-9
(Leu23-Asp185)
Accession # O00144		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu23

Predicted Molecular Mass

44 kDa

SDS-PAGE

54-63 kDa, reducing conditions

Activity

Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a functional ELISA with an ED50 of 15-90 ng/mL.

Formulation, Preparation and Storage

9175-FZ
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-9

Frizzled-9 (Fzd9, previously called Fzd3) belongs to the Frizzled family of seven-transmembrane glycoprotein receptors, which are signaling receptors for the family of secreted Wnt ligands (1-3). Mature human Frizzled-9 consists of a 207 amino acid (aa) N-terminal extracellular domain (ECD), the 7TM region, and a 62 aa C-terminal cytoplasmic domain with a PDZ binding motif. The ECD includes a cysteine-rich region that binds Wnts and is highly conserved among Frizzleds. Within aa 23-185 of the N-terminal ECD, human Frizzled-9 shares 97% aa identity with mouse and rat Frizzled-9. The human Frizzled-9 gene maps to a large region that is deleted in Williams-Beuren syndrome, a developmental disorder affecting personality and cognition (2). Frizzled-9 mRNA is expressed in the central nervous system and myotomes during development, and it persists in the hippocampus throughout life (2-4). Adults also show expression in heart, testis, and skeletal muscle (2-4). Cellular expression is detected in proliferating neural precursors, B lymphocyte progenitors, differentiating osteoblasts, and developing myotubes of skeletal muscle (4-7). Mice lacking Frizzled-9 exhibit defects in learning, B lymphocyte development, and bone mineralization (4-6, 8). Frizzled-9 can signal through both canonical and non-canonical pathways (6, 9). Its binding to Wnt-7a promotes the activation of PPAR gamma, expression of Cadherin and Sprouty, and reversal of non-small cell lung cancer cell transformation (10-12).

References

  1. Dijksterhuis, J.P. et al. (2014) Br. J. Pharmacol. 171:1195.
  2. Wang, Y.K. et al. (1997) Hum. Mol. Genet. 6:465.
  3. Wang, Y.K. et al. (1999) Genomics 57:235.
  4. Zhao, C. et al. (2005) Development 132:2917.
  5. Ranheim, E.A. et al. (2005) Blood 105:2487.
  6. Albers, J. et al. (2011) J. Cell Biol. 192:1057.
  7. Aviles, E.C. et al. (2014) Front. Cell. Neurosci. 8:110.
  8. Heilmann, A. et al. (2013) PLoS One 8:e84232.
  9. Karasawa, T. et al. (2002) J. Biol. Chem. 277:37479.
  10. Winn, R.A. et al. (2005) J. Biol. Chem. 280:19625.
  11. Winn, R.A. et al. (2006) J. Biol. Chem. 281:26943.
  12. Tennis, M.A. et al. (2010) Mol. Cancer Res. 8:833.

Alternate Names

CD349, Frizzled9, FZD9

Entrez Gene IDs

8326 (Human); 14371 (Mouse)

Gene Symbol

FZD9

UniProt

O00144

Additional Frizzled-9 Products

Product Documents for Recombinant Human Frizzled-9 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human Frizzled-9 Fc Chimera Protein, CF

For research use only

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