Recombinant Human Fucosyltransferase 11/FUT11 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5964-GT

R&D Systems, part of Bio-Techne
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5964-GT-020
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Key Product Details

Learn more about Fluorescent Glycan Labeling and Detection

Source

CHO

Accession #

AAH36037

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Fucosyltransferase 11/FUT11 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Fucosyltransferase 11/FUT11 protein
Gly25-Leu492, with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

54 kDa

SDS-PAGE

65-73 kDa, reducing conditions

Activity

Measured by its ability to hydrolyze the donor substrate GDP-fucose.
The specific activity is >4 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

5964-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Fucosyltransferase 11/FUT11

Fucose is frequently found at terminal sites on various glycans and is essential for the generation of many sugar epitopes. Well-known fucose containing glycans include Lewis and ABO blood group antigens. Lewis epitopes are key elements involved in leukocyte homing and extravasation process, thus are essential for lymphocyte maturation and natural defense functions (1). O-fucosylation on Notch receptor is found to be essential for its signaling function (2). So far, 11 carbohydrate specific fucosyltransferases are found in humans (3). FUT1 and FUT2 are alpha1-2 fucosyltransferases and are responsible for ABO blood group antigen synthesis (4). FUT3, FUT4, FUT5, FUT6, FUT7 and FUT9 are alpha1-3/4 fucosyltransferases and are responsible for Lewis antigen generation (5). FUT10 and FUT11 are newly cloned alpha1-3 fucosyltransferases that are distinct from the alpha1-3/4 fucosyltransferase subfamily and are able to introduce fucose to the innermost core GlcNAc of the N-glycan on conalbumin glycopeptides and biantennary N-glycan acceptors but not onto short lactosaminyl acceptor substrates (6). Predicted as a type II transmembrane protein and a Golgi resident enzyme, the exact function of this enzyme needs to be further characterized. The activity of this enzyme has been measured with a phosphatasecoupled method (7).

References

  1. Weston, B. W. et al. (1992) J. Biol.Chem. 267:4152.
  2. Stahl, M. et al. (2008) J. Biol.Chem. 283:13638.
  3. Becker, D.J. et al. (2003) Glycobiology 13:41R.
  4. Kelly, R. J. et al. (1995) J. Biol.Chem. 270:4640.
  5. Weston, B. W. et al. (1992) J. Biol.Chem. 267:24575.
  6. Mollicone, R. et al. (2009) J. Biol. Chem. 284:4723.
  7. Wu, Z.L. et al. (2011) Glycobilogy 21:727.

Alternate Names

Fuc-TXI, FUT11

Entrez Gene IDs

170384 (Human); 73068 (Mouse); 286971 (Rat)

Gene Symbol

FUT11

UniProt

AAH36037

Additional Fucosyltransferase 11/FUT11 Products

Product Documents for Recombinant Human Fucosyltransferase 11/FUT11 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Fucosyltransferase 11/FUT11 Protein, CF

For research use only

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