Recombinant Human GDF-1 Protein

R&D Systems, part of Bio-Techne | Catalog # 6937-GD

R&D Systems, part of Bio-Techne
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Carrier Free
6937-GD-010/CF
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With Carrier
6937-GD-010
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Key Product Details

Source

CHO

Accession #

NP_001483

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all GDF-1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human GDF-1 protein
Asp254-Arg372

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp254

Predicted Molecular Mass

12.6 kDa (monomer)

SDS-PAGE

13 kDa, reducing conditions

Activity

Measured by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:653.
50 ng/mL of Recombinant Human GDF‑1 can effectively induce Smad2 phosphorylation.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 6937-GD
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 6937-GD/CF
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-1

Growth and Differentiation Factor 1 (GDF‑1) is a 12 ‑ 15 kDa secreted protein in the TGF‑ beta superfamily (1). It is the functional equivalent of frog, zebrafish, and chick Vg1 (2, 3). Human GDF‑1 is synthesized as a preproprotein that contains a 29 amino acid (aa) signal peptide, a 224 aa propeptide, and a 119 aa mature peptide which is released following proteolysis at a dibasic cleavage site (4). Mature human GDF‑1 shares 81% aa sequence identity with mouse and rat GDF‑1. GDF‑1 is expressed in the developing and adult nervous system, particularly in neurons of the cerebral cortex and hippocampus (4, 5). It is required during embryogenesis for development of left/right axis asymmetry, anterior/posterior axis asymmetry, and the notochord (2, 3, 6, 7). It cooperates with Nodal and GDF‑3 in these activities and potentiates Nodal bioactivity through formation of a 27 kDa noncovalent GDF‑1‑Nodal heterodimer (3, 7, 8). The presence of Nodal is required for GDF‑1 induced effects (8). GDF‑1 signals through heteromeric receptor complexes containing Activin RIIA or Activin RIIB, ALK4 or ALK7, and an EGF‑CFC protein such as Cryptic or Cripto (7 ‑ 9). Loss of function mutations in GDF‑1 are associated with a variety of human cardiac development defects (10).

References

  1. Rider, C.C. and B. Mulloy (2010) Biochem. J. 429:1.
  2. Wall, N.A. et al. (2000) Dev. Biol. 227:495.
  3. Andersson, O. et al. (2007) Dev. Biol. 311:500.
  4. Lee, S.-J. (1991) Proc. Natl. Acad. Sci. 88:4250.
  5. Soderstrom, S. and T. Ebendal (1999) J. Neurosci. Res. 56:482.
  6. Rankin, C.T. et al. (2000) Nat. Genet. 24:262.
  7. Andersson, O. et al. (2006) Dev. Biol. 293:370.
  8. Tanaka, C. et al. (2007) Genes Dev. 21:3272.
  9. Cheng, S.K. et al. (2003) Genes Dev. 17:31.
  10. Karkera, J.D. et al. (2007) Am. J. Hum. Genet. 81:987.

Long Name

Growth Differentiation Factor 1

Alternate Names

GDF1

Entrez Gene IDs

2657 (Human); 14559 (Mouse)

Gene Symbol

GDF1

UniProt

NP_001483

Additional GDF-1 Products

Product Documents for Recombinant Human GDF-1 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 6937-GD (with carrier)
Download SDS 6937-GD/CF (carrier free)

Product Specific Notices for Recombinant Human GDF-1 Protein

For research use only

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