Recombinant Human GIF Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8387-TC

R&D Systems, part of Bio-Techne
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8387-TC-050
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Key Product Details

Source

HEK293

Accession #

P27352

Conjugate

Unconjugated

Applications

Bioactivity

View all GIF Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human GIF protein
Ser19-Tyr417 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser19

Predicted Molecular Mass

44 kDa

SDS-PAGE

44-60 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

8387-TC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in sterile water.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GIF

Gastric Intrinsic Factor (GIF) also known as Intrinsic Factor (IF) is a 45-55 kDa glycoprotein that is secreted by parietal cells of the gastric mucosa. GIF is a member of the eukaryotic cobalamin (Cbl or Vitamin B12) family of transport proteins that mediate the uptake of cobalamin by intestinal epithelial cells lining the ileum (1). GIF comprises two domains, an alpha and a beta-domain containing approximatively 270 and 110 residues respectively (2). Cobalamin binds to GIF at the interface of the alpha and beta domains (2). The Cbl-GIF complex can then interact with its specific receptor, Cubilin, through the amino-terminal portion of GIF (3). This complex associates with Amnionless (AMN) which directs subcellular localization and endocytosis of Cubilin and its ligands (4). Homozygous nonsense and missense mutations in the GIF gene have been linked to hereditary juvenile megaloblastic anemia. These patients show nutritional cobalamin insufficiency due to its intestinal malabsorption (5). Mature human GIF shares 82% and 79% amino acid sequence identity with mouse GIF and rat GIF respectively.

References

  1. Hewitt, J.E. et al. (1991) Genomics 10:432.
  2. Mathews, F.S. et al. (2007) Proc. Natl. Acad. Sci. USA 104:17311.
  3. Tang, L-H. et al. (1992) J. Biol. Chem. 267:22982.
  4. Fyfe, J.C. et al. (2004) Blood 103:1573.
  5. Tanner, S.M. et al. (2005) Proc. Natl. Acad. Sci. USA 102:4130.

Long Name

Gastric Intrinsic Factor

Alternate Names

IF, IFMH, INF, TCN3

Entrez Gene IDs

2694 (Human); 14603 (Mouse); 29319 (Rat)

Gene Symbol

CBLIF

UniProt

P27352

Additional GIF Products

Product Documents for Recombinant Human GIF Protein, CF

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Product Specific Notices for Recombinant Human GIF Protein, CF

For research use only

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