Recombinant Human Glypican 3 Fc Chimera Avi-tag Protein, CF

Name: Recombinant Human Glypican 3 Fc Chimera Avi-tag Protein, CF
Brand: R&D Systems
SKU: AVI11078

R&D Systems, part of Bio-Techne | Catalog # AVI11078

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Product Datasheet / COA / SDS

Key Product Details

Source:	CHO
Structure / Form:	Disulfide linked homodimer Biotinylated via Avi-tag
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Glypican 3 Fc Chimera Avi-tag Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human Glypican 3 Fc Chimera Avi-tag Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived human Glypican 3 protein

Human Glypican-3 (Gln25-His559) Accession # P51654.1	IEGRMD	Human IgG1 (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Gln25

Predicted Molecular Mass

89 kDa

SDS-PAGE

89 kDa

Activity

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human Glypican 3 Fc Chimera Avi-tag (Catalog # AVI11078) binds to Recombinant Human FGF basic/FGF2/bFGF (Catalog # 233-FB/CF) with an ED₅₀ of 60.0-720 ng/mL.

Scientific Data Examples for Recombinant Human Glypican 3 Fc Chimera Avi-tag Protein, CF

	Biotinylated Recombinant Human Glypican 3 Fc Chimera Avi-tag Protein Binding Activity. In a functional ELISA, Biotinylated Recombinant Human Glypican 3 Fc Chimera Avi-tag Protein (Catalog # AVI11078) binds to Recombinant Human FGF basic/FGF2/bFGF (233-FB/CF) with an ED₅₀ of 60.0-720 ng/mL.
	Biotinylated Recombinant Human Glypican 3 Fc Chimera Avi-tag Protein SDS-PAGE. 2 μg/lane of Biotinylated Recombinant Human Glypican 3 Fc Chimera Avi-tag Protein (Catalog # AVI11078) was resolved with SDS-PAGE under non-reducing (NR) condition and visualized by Coomassie® Blue staining, showing bands at >190 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11078

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Glypican 3

Glypican 3 (GPC3), also known as OCI5 and MXR7, is a member of the heparan sulfate proteoglycan (HSPG) family (1). In mammals, six glypican family members have been identified, all sharing a structurally common extracellular domain (ECD) with a large globular cysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C‑terminal GPI attachment site. The ECD of GPC3 can be cleaved by furin to produce two subunits that are linked by disulfide bonds: a 40 kDa N‑terminal alpha subunit that can be secreted into the blood and a 30 kDa membrane-bound C‑terminal beta subunit containing two HS glycan chains (1-3). Within ECD, human GPC3 shares 96% amino acid sequence identity with both mouse and rat GPC3. Several isoforms of GPC3 due to alternative splicing have been reported (1). GPC3 is widely expressed on the membrane of various embryonic cells, but not on those in adult liver, and is involved in the regulation of growth and development of the body (4). GPC3 is over-expressed in hepatocellular carcinomas and binding of GPC3 to CD81 promotes development of carcinomas by activation of Hippo pathways in hepatocytes (5). GPC3 is an important biomarker present in the serum of hepatocellular carcinoma patients, which distinguishes benign from cancerous nodules (6). Loss-of-function mutations in GPC3 are associated with Simpson-Golabi-Behmel (SGB) syndrome, a condition characterized by tissue overgrowth (dysmorphogenesis) (7). Our Avi-tag Biotinylated Recombinant Glypican 3 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

Item	Value
References	Ho, M. and Kim, H. (2012) Eur. J. Cancer. 47:333. Haruyama, Y. and Kataoka, H. (2016) World J. Gastroenterol. 22:275. Shimizu, Y. et al. (2019) Front Oncol. 9:248. Gonzales, A.D. et al. (1998) J. Cell Biol. 141:1407. Xue, Y. et al. (2018) Am J Pathol. 188(6):1469. Ge, S. et al. (2018) Filmus, Int J Clin Exp Pathol. 11(12):5774. Pilia, G. et al. (1996) Nature Genet.12:241.
Entrez Gene IDs	2719 (Human); 14734 (Mouse); 25236 (Rat); 102137748 (Cynomolgus Monkey)
Alternate Names	DGSX, GPC3, GTR2-2, Glypican 3, Intestinal protein OCI-5, MXR7, OCI-5, OCI5, SGB, SGBS, SGBS1SDYS, glypican proteoglycan 3, glypican-3, heparan sulphate proteoglycan, secreted glypican-3

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Recombinant Human Glypican 3 Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11078

Key Product Details

Product Specifications for Recombinant Human Glypican 3 Fc Chimera Avi-tag Protein, CF

Scientific Data Examples for Recombinant Human Glypican 3 Fc Chimera Avi-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Glypican 3

Customer Reviews for Recombinant Human Glypican 3 Fc Chimera Avi-tag Protein, CF

Product Documents for Recombinant Human Glypican 3 Fc Chimera Avi-tag Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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