Recombinant Human Glypican 3 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11078-GP

R&D Systems, part of Bio-Techne
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Key Product Details

Source

HEK293

Accession #

P51654.1

Structure / Form

Disulfide-linked homodimer. Glypican 3 is subject to endoproteolytic processing by proprotein convertases (PC). By amino acid sequencing, Two subunits (Alpha and Beta) are present (Alpha inferred to start with Gln25 and Beta starting with Ser359).  They are associated via disulfide bonds.   

Conjugate

Unconjugated

Applications

Bioactivity

View all Glypican 3 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Glypican 3 protein
Human Glypican 3
(Gln25-His559)
Accession # P51654.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln25 inferred from enzymatic pyroglutamate treatment revealing Pro26 and Ser359.

Predicted Molecular Mass

87 kDa (monomer)

SDS-PAGE

230-330 kDa, under non-reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human FGF basic/FGF2/bFGF (Catalog # 233-FB/CF) is immobilized at 0.500 µg/mL (100 µL/well), Recombinant Human Glypican 3 Fc Chimera Protein binds with an ED50 of 0.0120-0.120 µg/mL.

Scientific Data Images for Recombinant Human Glypican 3 Fc Chimera Protein, CF

Recombinant Human Glypican 3 Fc Chimera Protein Binding Activity.

When Recombinant Human FGF basic/FGF2/bFGF (233-FB) is immobilized at 0.500 µg/mL (100 µL/well), Recombinant Human Glypican 3 Fc Chimera Protein (Catalog # 11078-GP) binds with an ED50 of 0.0120-0.120 µg/mL.

Formulation, Preparation and Storage

11078-GP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glypican 3

Glypican 3 (GPC3), also known as OCI5 and MXR7, is a member of the heparan sulfate proteoglycan (HSPG) family (1). In mammals, six glypican family members have been identified, all sharing a structurally common extracellular domain (ECD) with a large globular cysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site. The ECD of GPC3 can be cleaved by furin to produce two subunits that are linked by disulfide bonds: a 40 kDa N-terminal alpha subunit that can be secreted into the blood and a 30 kDa membrane-bound C-terminal beta subunit containing two HS glycan chains (1-3). Within ECD, human GPC3 shares 96% amino acid sequence identity with both mouse and rat GPC3. Several isoforms of GPC3 due to alternative splicing have been reported (1). GPC3 is widely expressed on the membrane of various embryonic cells, but not on those in adult liver, and is involved in the regulation of growth and development of the body (4). GPC3 is over-expressed in hepatocellular carcinomas and binding of GPC3 to CD81 promotes development of carcinomas by activation of Hippo pathways in hepatocytes (5). GPC3 is an important biomarker present in the serum of hepatocellular carcinoma patients, which distinguishes benign from cancerous nodules (6). Loss-of-function mutations in GPC3 are associated with Simpson-Golabi-Behmel (SGB) syndrome, a condition characterized by tissue overgrowth (dysmorphogenesis) (7).

References

  1. Ho, M. and Kim, H. (2012) Eur. J. Cancer. 47:333.
  2. Haruyama, Y. and Kataoka, H. (2016) World J. Gastroenterol. 22:275.
  3. Shimizu, Y. et al. (2019) Front Oncol. 9:248.
  4. Gonzales, A.D. et al. (1998) J. Cell Biol. 141:1407.
  5. Xue, Y. et al. (2018) Am J Pathol. 188(6):1469.
  6. Ge, S. et al. (2018) Filmus, Int J Clin Exp Pathol. 11(12):5774.
  7. Pilia, G. et al. (1996) Nature Genet.12:241.

Alternate Names

GPC3

Entrez Gene IDs

2719 (Human); 14734 (Mouse); 25236 (Rat); 102137748 (Cynomolgus Monkey)

Gene Symbol

GPC3

UniProt

P51654.1

Additional Glypican 3 Products

Product Documents for Recombinant Human Glypican 3 Fc Chimera Protein, CF

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