Recombinant Human GPIHBP1 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10091-GB

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
10091-GB-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

HEK293

Accession #

Q8IV16

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all GPIHBP1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human GPIHBP1 protein
Human GPIHBP1
(Thr22-Gly151)
Accession # Q8IV16		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr22

Predicted Molecular Mass

41 kDa

SDS-PAGE

52-63 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Lipoprotein Lipase (Catalog # 9888-LL) is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Human GPIHBP1 Fc Chimera that produces 50% optimal binding response is 1.5-9 μg/mL.

Scientific Data Images for Recombinant Human GPIHBP1 Fc Chimera Protein, CF

Recombinant Human GPIHBP1 Fc Chimera Protein Binding Activity

Recombinant Human GPIHBP1 Fc Chimera Protein Binding Activity

When Recombinant Human Lipoprotein Lipase (Catalog # 9888-LL) is coated at 2 µg/mL,100 µL/well, Recombinant Human GPIHBP1 Fc Chimera (Catalog # 10091-GB) binds with an ED50 of 1.5-9 µg/mL.
Recombinant Human GPIHBP1 Fc Chimera Protein SDS-PAGE

Recombinant Human GPIHBP1 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human GPIHBP1 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 52-63 kDa and 100-130 kDa, respectively.

Formulation, Preparation and Storage

10091-GB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: GPIHBP1

GPIHBP1 (Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1) is a member of the Ly6 family of proteins (1, 2). Human GPIHBP1 is synthesized as a 184 amino acid (aa) protein that includes a 20 aa signal peptide, a 131 aa GPIHBP1 chain containing the Ly6 domain, and a 33 aa propeptide. Within the main chain region, human GPIHBP1 shares 54% and 53% aa sequence identity with mouse and rat GPIHBP1, respectively. It is found on capillaries of heart, skeletal muscle, and adipose tissue (3), but not expressed in endothelial cells of larger blood vessels or expressed in capillaries of the brain (3, 4).  GPIHBP1 binds both lipoprotein lipase (LPL) and chylomicrons (CM), and the acidic domain of GPIHBP1 is required for LPL binding (3, 5). Triglyceride-rich lipoproteins undergo lipolysis by LPL bound by GPIHBP1 and transported across the cells to the capillary lumen (6-7). GPIHBP1 is an important regulator of triglyceride metabolism by increasing the efficiency of lydrolysis by LPL and uptake of fatty acids (3, 8-9).

References

  1. Young, S.G. et al. (2011) J. Lipid Res. 52:1869.
  2. Ioka, R.X. et al. (2003) J. Biol. Chem. 278:7344.
  3. Beigneux, A.P. et al. (2007). Cell Metab. 5:279.
  4. Davies, B.S.J. et al. (2010) Cell Metab. 12:42.
  5. Gin, P. (2008) J. Biol. Chem. 283:29554.
  6. Davies, B.S. et al. (2012) J. Lipid Res. 53:2690.
  7. Goulborne, C.N. et al. 2014 Cell Metabolism 19:849.
  8. Young, S.G. et al. (2007) Curr. Opin. Lipidol. 18:389.
  9. Sonneburg, W.K. et al. (2009) J Lipid Research 50:2421.

Long Name

Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1

Alternate Names

GPI-Anchored HDL-Binding Protein 1, GPI-HBP1, HBP1, HYPL1D

Entrez Gene IDs

338328 (Human); 68453 (Mouse); 300027 (Rat)

Gene Symbol

GPIHBP1

UniProt

Q8IV16

Additional GPIHBP1 Products

Product Documents for Recombinant Human GPIHBP1 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human GPIHBP1 Fc Chimera Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.