Recombinant Human GPR114 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4747-GP
Key Product Details
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human GPR114 protein
Glu22-Gly184 , with a C-terminal 6-His tag
Glu22-Gly184 , with a C-terminal 6-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Glu22
Predicted Molecular Mass
19.4 kDa
SDS-PAGE
40-55 kDa, reducing conditions
Activity
Measured by the ability of the immobilized protein to enhance the adhesion of T98G human glioblastoma cells to human Fibronectin.
When 4 x 104 cells/well are added to plates coated with human Fibronectin (0.1 µg/mL, Catalog # 1918-FN) and rhGPR114 (10 µg/well, 100 µL/well) for 60 minutes at 37° C, there is a 2-4 fold increase in adhesion to human fibronectin induced by the presence of rhGPR114.
Optimal dilutions should be determined by each laboratory for each application.
When 4 x 104 cells/well are added to plates coated with human Fibronectin (0.1 µg/mL, Catalog # 1918-FN) and rhGPR114 (10 µg/well, 100 µL/well) for 60 minutes at 37° C, there is a 2-4 fold increase in adhesion to human fibronectin induced by the presence of rhGPR114.
Optimal dilutions should be determined by each laboratory for each application.
Formulation, Preparation and Storage
4747-GP
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: GPR114
GPR114 is a member of the LN-TM7 family of adhesion-type 7-transmembrane (TM) G-protein coupled receptors (GPCR) that exhibit long extracellular N-termini (1 - 3). The 528 amino acid (aa) human GPR114 sequence predicts a 21 aa signal sequence, a 229 aa N-terminal extracellular domain (ECD) and seven TM regions separated by short intracellular and extracellular regions. Like other LN-TM7 members, the ECD contains a highly glycosylated mucin-like stalk that is predicted to function in adhesion, followed by a GPCR proteolytic cleavage site (GPS) (2); it does not contain any other easily recognizable domains. Adhesion ligands have been reported for only a few LN-TM7 members (1). GPR114 is not one of them. Human GPR114 aa 22 - 184 share 63%, 63%, 67% and 59% aa identity with the corresponding regions of mouse, rat, bovine and equine GPR114, respectively. GPR114 was identified from expressed sequence tags (ESTs) found in leukemia cells, leukocytes, and pancreatic islets cells (2). An alternative start site has also been reported at Met 233 (4). One functional splice variant of GPR114 has been identified in colon, leukocytes, spleen and thymus (3).
References
- Bjarnadottir, T.K. et al. (2007) Cell. Mol. Life Sci. 64:2104.
- Fredriksson, R. et al. (2002) FEBS Lett. 531:407.
- Bjarnadottir, T.K. et al. (2007) Gene 387:38.
- Accession # EAW82937.
Long Name
G Protein-coupled Receptor 114
Alternate Names
PGR27
Gene Symbol
ADGRG5
UniProt
Additional GPR114 Products
Product Documents for Recombinant Human GPR114 Protein, CF
Product Specific Notices for Recombinant Human GPR114 Protein, CF
For research use only
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