Recombinant Human GPR115 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 5437-GP
Key Product Details
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human GPR115 protein
Ser22-Ala347, with a C-terminal 6-His tag
Ser22-Ala347, with a C-terminal 6-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser22
Predicted Molecular Mass
37 kDa
SDS-PAGE
66-100 kDa, reducing conditions
Activity
Measured by the ability of the immobilized protein to support the adhesion of U-87 MG human glioblastoma/astrocytoma cells.
When 5 x 104 cells per well are added to rhGPR-115 coated plates (5 μg/mL, 100 μL/well), approximately 60-85% will adhere after 60 minutes at 37 °C.
When 5 x 104 cells per well are added to rhGPR-115 coated plates (5 μg/mL, 100 μL/well), approximately 60-85% will adhere after 60 minutes at 37 °C.
Formulation, Preparation and Storage
5437-GP
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 200 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: GPR115
GPR115 is a member of the LN-7TM family of adhesion-type 7-transmembrane (TM) G-protein coupled receptors (GPCR) that show a long extracellular N-terminus (1, 2). The 695 amino acid (aa) human GPR115 sequence predicts a 21 aa signal sequence, a 385 aa N-terminal extracellular domain (ECD), seven TM regions separated by 6 - 24 aa intracellular and extracellular regions, and a 40 aa cytoplasmic tail. Like other LN-7TM members, the ECD contains a highly glycosylated mucin-like stalk that is predicted to function in adhesion. This is followed by a cysteine-rich GPCR proteolytic cleavage site (GPS) (1). GPS domains, which have been described in other 7TM proteins including ETL, GPR126, HE6, and Latrophilin-1, are cleavage sites for processing proteins into two subunits (3 - 7). Within the N terminal region that ends with the predicted cleavage site (aa 22 - 347), human GPR115 shares 58% aa sequence identity with the corresponding region of mouse and rat GPR115. GPR115 was identified from expressed sequence tags (ESTs) found in pregnant uterus, breast, and the genitourinary tract (1).
References
- Fredriksson, R. et al. (2002) FEBS Lett. 531:407.
- Bjarnadottir, T.K. et al. (2004) Genomics 84:23.
- Nechiporuk, T. et al. (2001) J. Biol. Chem. 276:4150.
- Moriguchi, T. et al. (2004) Genes Cells 9:549.
- Kierszenbaum, A.L. (2003) Mol. Reprod. Dev. 64:1.
- Krasnoperov, V.G. et al. (1997) Neuron 18:925.
- Krasnoperov, V. et al. (2002) J. Biol. Chem. 277:46518.
Long Name
G Protein-coupled Receptor 115
Alternate Names
PGR18
Gene Symbol
ADGRF4
UniProt
Additional GPR115 Products
Product Documents for Recombinant Human GPR115 Protein, CF
Product Specific Notices for Recombinant Human GPR115 Protein, CF
For research use only
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